Fluorescence microscopy core

荧光显微镜核心提供荧光显微镜技术方面的技术专长， 定量图像处理应用，包括光学切片和多维成像， 非线性光学显微镜（aka 2-光子成像），荧光共振能量转移（FRET），总 内反射荧光（TIRF）和定量活细胞荧光成像。提供的服务 核心包括获得最先进的仪器和软件并提供技术援助， 在荧光成像应用的实验设计和数据解释方面的专业知识。核心是 在Richard D。柏林细胞分析和建模中心（CCAM; www.ccam.uchc.edu）， 和血管生物学中心在同一层CCAM和 血管生物学中心使CCAM和程序之间的简单和广泛的互动 组 相关性（参见说明）： 荧光显微镜核心将协助PPG研究人员进行复杂的实验， 荧光显微镜和相关技术，实验的目的是可视化细胞在非常 高倍放大，以便更好地了解动脉粥样硬化的疾病过程。

The Fluorescence Microscopy Core provides technical expertise in fluorescence microscopy techniques and quantitative image processing applications, including optical sectioning and multi-dimensional imaging, nonlinear optical microscopy (aka 2-photon imaging), fluorescence resonance energy transfer (FRET), total internal reflection fluorescence (TIRF) and quantitative live-cell fluorescence imaging. Services provided by the core include access to and technical assistance with state of the art instrumentation and software, and expertise in experimental design and data interpretation for fluorescence imaging applications. The core is located at the Richard D. Berlin Center for Cell Analysis and Modeling (CCAM; www.ccam.uchc.edu), and is situated on the same floor as the Center for Vascular Biology. The close proximity between CCAM and the Center for Vascular Biology makes for easy and extensive interactions between CCAM and the program group. RELEVANCE (See instructions): The Fluorescence Microscopy Core will assist the PPG investigators with sophisticated experiments that use fluorescence microscopy and related techniques, The aim of the experiments are to visualize cells at very high magnification so that the disease process of atherosclerosis is better understood.