Purchase of AB Sciex QTRAP 5500 System

描述（由申请人提供）：该提案来自伊利诺伊大学芝加哥分校（UIC）的一组已建立的研究人员，旨在购买LC/MS/MS设备，完全致力于分析各种类型的脂质和脂质衍生的第二信使，这些脂质和脂质衍生的第二信使涉及几种人类疾病和疾病的病理生物学。申请资金用于购买连接到安捷伦HPLC系统的AB Sciex QTRAP 5500质谱仪。本应用程序的参与者包括13个主要用户和8个次要用户，他们是nih支持的活跃研究者，由大约50个R01或P01项目支持，其中至少30个将直接受益于该仪器。这些研究人员从事一些基础和临床相关的研究，包括细胞信号通路、心血管疾病、糖尿病、肥胖、肺部和呼吸系统疾病，他们需要一种专用的仪器来表征和量化组织、血浆、生物液体和培养细胞中的磷脂、鞘脂和中性脂的各种分子种类。进一步发展新的和更灵敏的生物活性脂质的测量需要现场可用的最先进的质谱仪。目前在UIC使用的LC/MS仪器要么不适合用于此目的，要么被充分利用

DESCRIPTION (provided by applicant): This proposal, from a group of established investigators at the University of Illinois at Chicago (UIC), is for the purchase of an LC/MS/MS equipment, fully dedicated to the analysis of various types of lipids and lipid-derived second messengers involved in pathobiology of several human disorders and diseases. Funds are requested for the purchase of an AB Sciex QTRAP 5500 mass spectrometer coupled to an Agilent HPLC system. Participants in this application include13 major users and 8 minor users who are active NIH-supported investigators, supported by about 50 R01 or P01 projects, of which at least 30 will be directly benefited by the instrument. These investigators are engaged in several basic and clinically relevant studies, including cell signaling pathways, cardiovascular diseases, diabetes, obesity, and pulmonary and respiratory disorders, and are in need of a dedicated instrument to characterize and quantify various molecular species of phospholipids, sphingolipids, and neutral lipids in tissues, plasma, biological fluids and cultured cells. Further development of new and more sensitive measurement of bioactive lipids requires on-site availability of state-of-the-art mass spectrometer. The LC/MS instruments that are currently available and in operation at UIC are either not suitable for this purpose, or being utilized fully for other types of analyses, such as proteins, peptides and drug metabolites. Therefore, availability of a state-of-the-art dedicated equipment for analysis of various types of lipids, lipd-derived second messengers and bioactive phospho- and sphingo-lipids will enable the currently NIH funded and non-funded investigators to take advantage of the novel lipidomic methods recently developed by the UIC faculty, as well as by others, towards enhancing their productivity and ability to conduct cutting edge translational research. PUBLIC HEALTH RELEVANCE: Lipids are an important and diverse group of fat molecules that are implicated in several human diseases, including heart and lung diseases, obesity, cancer, diabetes, and inflammatory diseases. The investigators in this application are engaged in several clinically relevant projects that require sophisticated lipid analysis. The knowledge gained from these studies could provide valuable tools for improved diagnosis and treatment of several of the above diseases.