Nikon A1RSI Confocal Microscope

描述(由申请人提供)：这是克利夫兰州立大学(CSU)的一个研究团队提出的共享仪器S10申请，要求购买尼康A1共焦显微镜。CSU目前还没有共焦显微镜。事实上，目前有一个单一的荧光显微镜，它是旧的，有限的使用和不共焦，由所有研究人员共享。所需设备的主要用户将是NIH资助的教职员工，他们主要来自两个部门，但都属于新成立的健康和疾病基因调控中心(GRHD)。少数用户将包括其他成员，他们中的大多数也有较小的NIH赠款和非NIH资金。总而言之，这是一群规模不大但非常活跃的研究人员，他们大多处于相对较早的职业生涯阶段，在各自的领域被誉为正在崛起的领导者。随着GRHD研究项目的进展和发展，我们感到显然需要高分辨率显微镜，而校园里缺乏这样的设施。GRHD的集体研究领域将受益于显微镜，至少包括：核糖体外L13a对翻译和rRNA甲基化的调节；锥虫端粒复合体的调节；内皮细胞药物输送；染色体结构和减数分裂期间的重组；生物钟位置和功能；弓形虫与宿主线粒体的相互作用；RNA病毒对先天性免疫的调节；巨核细胞和多能干细胞的分化；小RNA的生物发生和功能。虽然细节各不相同，但这些研究人员的总体目标是了解特定蛋白质的亚细胞定位如何调节细胞和致病过程，与心血管疾病、传染病、先天免疫、衰老和癌症相关。为了充分发挥这些项目的潜力，绝对需要一种能够自动聚焦、延时成像并在固定细胞和活细胞中分离多个荧光团的共焦显微镜，这一提议概述了这一点。此外，这所公立大学还将加强本科生和研究生的培训和教育。为了补充NIH的预算，CSU和科学与健康专业学院的各个办公室(系主任、学院院长和研究副总裁)将提供适当的空间，支持训练有素的人员操作显微镜，并在需要时购买延长的服务合同。 公共卫生相关性：在这一共享仪器应用中要求的尼康A1共聚焦显微镜，将加强克利夫兰州立大学新成立的健康和疾病基因调控中心的一支强大的研究团队的研究。受益于显微镜的多个研究领域将包括心血管和传染病、先天性疾病、衰老和癌症。

DESCRIPTION (provided by applicant): This is a Shared Instrumentation S10 application from a team of investigators at Cleveland State University (CSU), requesting a Nikon A1 Confocal Microscope. There is no confocal microscope in CSU at present. In fact, there is currently a single fluorescence microscope, which is old, limited-use and not confocal, shared by all research faculty. The major users of the requested equipment will be NIH-funded Faculty members, drawn mainly from two Departments, but all belonging to the newly created Center for Gene Regulation in Health and Disease ("GRHD"). Minor users will include other members, most of whom also have smaller NIH grants and non-NIH funding. Together, this is a small but highly active group of researchers, mostly in relatively early career stages, acclaimed as rising leaders in their respective fields. As the research projects of GRHD progressed and evolved, we felt a clear need for high-resolution microscopy, contrasted by the lack of such a facility on campus. The collective research areas of GRHD, benefiting from the microscope, will minimally include: Regulation of translation and rRNA methylation by extra-ribosomal L13a; Regulation of Trypanosome telomere complex; Endothelial cell drug delivery; Chromosome structure and recombination during meiosis; Circadian clock location and function; Toxoplasma-host mitochondria interaction; Regulation of innate immunity by RNA viruses; Differentiation of megakaryocytes and pluripotent stem cells; Small RNA biogenesis and function. Although diverse in detail, the overall Aim of these investigators is to understand how the subcellular localization of specific proteins regulate cellular and pathogenic processes, relevant to cardiovascular disorders, infectious diseases, innate immunity, aging, and cancer. A confocal microscope capable of autofocus, time-lapse imaging and segregating multiple fluorophores in both fixed and living cells is absolutely needed to realize the full potential of these projects, a outlined within this proposal. An added benefit will be the enhancement of student training and education at both under- and post-graduate levels in this public University. To supplement the NIH budget, the various offices of CSU and the College of Sciences and Health Professions (Department Chairs, Dean of College, and Vice President of Research) will provide appropriate space, support trained personnel to operate the microscope, and purchase an extended service contract as and when needed. PUBLIC HEALTH RELEVANCE: The Nikon A1 Confocal Microscope, requested in this Shared Instrumentation application, will enhance the research of a strong team of investigators belonging to the newly created Center for Gene Regulation in Health and Disease at Cleveland State University. The multiple research areas benefiting from the microscope will include cardiovascular and infectious diseases, inborn disorders, aging and cancer.