EVALUTATION OF HUMAN STEM CELL ENGRAFTMENT TO SHIVERER MICE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目是谢尔曼实验室和干细胞公司的合作项目，重点是测试人类神经干细胞移植到寒战小鼠体内是否可以促进功能性的髓鞘再生。小鼠将被移植到干细胞公司的实验室，然后运往谢尔曼实验室。然后，将从小鼠的大脑中解剖胼胝体，并在神经科学部的电生理学核心设施中测试复合动作电位(CAPS)。记录后，将对组织进行染色，以确定是否存在少突胶质细胞和髓鞘。这些研究将证明一种新的人类神经干细胞培养系统在治疗脱髓鞘疾病方面的有效性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project is a collaborative effort between the Sherman lab and Stem Cells Inc. focused on testing whether human neural stem cells transplanted into shiverer mice can promote functional remyelination. Mice will be transplanted in the laboratories at Stem Cells Inc. then shipped to the Sherman lab. The corpus callosum will then be dissected from the brains of mice and tested for compound action potentials (CAPs) in the electrophysiology core facility in the Division of Neuroscience. Following recordings, tissues will be stained for the presence of oligodendrocytes and myelin. These studies will demonstrate the efficacy of a novel human neural stem cell culture system for the treatment of demyelinating diseases.