CTL EXCLUSION FROM LYMPHOID FOLLICLES AND LENTIVIRUS IMMUNE EVASION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 目的：研究慢性SIVmac 239感染恒河猴淋巴组织中病毒特异性CTL和病毒产生细胞分布的潜在机制，并确定慢病毒特异性CTL活性是否仅限于体内淋巴组织的滤泡外区室。 在HIV（人类免疫缺陷病毒）感染期间，引起大量的细胞毒性T细胞。这种细胞群的作用是消除病毒感染的细胞。然而，尽管血液中存在大量HIV特异性细胞毒性T细胞，但病毒在淋巴结的某些部分中非常有效地增殖。我们假设淋巴结的某些部分是免疫赦免的，细胞毒性T淋巴细胞（CTL）无法进入这些部位。因此，CTL可以杀死淋巴结某些区域的感染细胞，但不能杀死其他区域。在这项初步研究中，我们将感染三只动物，以确定慢性SIVmac 239感染恒河猴淋巴组织中病毒特异性CTL和病毒产生细胞的分布，并确定慢病毒特异性CTL活性是否仅限于体内淋巴组织的滤泡外区室。 进展： 我们已经从选定的动物中建立了B-LCL细胞系。我们正在建立CD 8 + T细胞系。 这项研究使用了WNPRC Animal Services、CPI和Immunology Services。 出版物： 没有。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To investigate potential mechanisms underlying the distribution of virus-specific CTL and virus-producing cells in lymphoid tissues of chronically SIVmac239-infected rhesus macaques and determine whether lentivirus-specific CTL activity is restricted to the extrafollicular compartment of lymphoid tissues in vivo. During HIV (human immunodeficiency virus) infection, an enormous number of cytotoxic T cells are elicited. The role of this cell population is to eliminate virally infected cells. However in spite the large number of HIV specific cytotoxic T cells in the blood, the virus proliferates very efficiently in some part of the lymph nodes. We hypothesize that certain parts of the lymph nodes are immune privileged and cytotoxic T lymphocytes (CTL) are unable to enter to these sites. As a consequence, CTL can kill infected cells in some areas of the lymph nodes, but not others. In this pilot study we are going to infect three animals to determine the distribution of virus-specific CTL and virus-producing cells in lymphoid tissues of chronically SIVmac239-infected rhesus macaques and determine whether lentivirus-specific CTL activity is restricted to the extrafollicular compartment of lymphoid tissues in vivo. PROGRESS: We have established a B-LCL cell line from a selected animal. We are in the process of establishing CD8+ T cell lines. This research used WNPRC Animal Services, CPI and Immunology Services. PUBLICATIONS: None.