IN VIVO EVALUATION OF PRIMATE LENTIVIRUSES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 当慢病毒株可用时，重要的是确定每种病毒在特定非人灵长类物种中的感染性，致病性和最小感染剂量，然后才能用于疫苗试验或治疗试验。 该项目旨在允许在体内测试慢病毒。 猿猴-人类免疫缺陷病毒-SF 162 P4（SHIV SF 162 P4）来源于分子克隆SHIV SF 162，这是一种嵌合病毒，含有B亚型HIV-1的CCR-5-使用原代分离株的env基因。 发现猕猴传代的原种SHIV SF 162 P3引起肠T细胞的急剧损失，随后逐渐耗尽外周T细胞。 在人PBMC中扩增来自感染SHIV SF 162 P3病毒两周后感染的猕猴的淋巴结细胞和PBMC，以产生P4储备病毒，并制备攻击储备物。 目前的研究进行测量的感染性和诱导能力的B-和T-细胞免疫反应的阴道内途径，使这些SHIV可以作为挑战病毒在未来的疫苗研究和杀微生物剂试验中，中国恒河猴利用这种感染途径。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. When strains of lentiviruses become available, it is important to determine the infectivity, pathogenicity, and minimal infectious dose of each virus in a particular nonhuman primate species before it can be used in vaccine trails or therapeutic testing. This project is designed to allow lentiviruses to be tested in vivo. Simian-Human Immunodeficiency Virus-SF162P4 (SHIV SF162P4) is derived from a molecular clone, SHIV SF162, a chimeric virus that contains the env gene of a CCR-5-using primary isolate of subtype B HIV-1. A macaque-passaged stock, SHIV SF162P3 was found to cause a dramatic loss of intestinal T cells followed by a gradual depletion of peripheral T cells. Lymph node cells and PBMC from a macaque infected with the SHIV SF162P3 virus two weeks after infection were amplified in human PBMC to generate a P4 stock virus and a challenge stock was prepared. The current study was undertaken to measure the infectivity and inductive ability of B- and T-cell immune responses by the intravaginal route so that these SHIVs may serve as challenge viruses in future vaccine studies and microbicide trials in Chinese rhesus macaques utilizing this route of infection.