Detection of Prions

朊病毒蛋白的病理亚型（PrPres）可以作为朊病毒疾病的标志物，但需要更多的实用测试用于临床前诊断和敏感检测许多朊病毒感染。在2011财年，我们1）开发了一种新的朊病毒检测方法（称为eQuIC），其允许超灵敏和快速地检测载瘤生物流体如血浆中的朊病毒; 2）使eQuIC适于检测血浆样品中的人、绵羊、仓鼠和小鼠朊病毒; 3）与人类TSE监测组进行培训和合作，以评估我们先前开发的RT-QuIC测定法用于诊断人类散发性克罗伊茨费尔特氏病的实用性。雅各布病;数据显示，当应用于临床脑脊髓液样品时，RT-QuIC具有比任何现有诊断测试更好的灵敏度和特异性。

The pathological isoform of the prion protein (PrPres) can serve as a marker for prion diseases, but more practical tests are needed for preclinical diagnosis and sensitive detection of many prion infections. During FY2011, we 1) developed a new prion assay (called eQuIC) that allows for the ultrasensitive and rapid of prions in inhibitor-laden biological fluids such as blood plasma; 2) adapted eQuIC to the detection of human, sheep, hamster and mouse prions in blood plasma samples; 3) trained and collaborated with human TSE surveillance groups to evaluate the utility of our previously developed RT-QuIC assay for the diagnosis of human sporadic Creutzfeldt-Jakob disease; the data showed that when applied to clinical cerebral spinal fluid samples, RT-QuIC has better sensitivity and specificity than any prior diagnostic test.