Regulation of podocyte function by hic-5

描述（由申请人提供）：足细胞足突（FP）和插入的狭缝隔膜（SD）形成蛋白质损失的最终屏障，解释了为什么足细胞损伤通常与明显的蛋白尿相关。以FP消失、SD破坏和蛋白尿为代表的足细胞功能障碍通常是进行性肾病的起点。在这里，我们建议测试我们的中心假设，即在足细胞中的hic-5表达的诱导有助于蛋白尿的发病机制，通过增加足细胞的细胞外基质（ECM）/肾小球基底膜（GBM）。我们进一步假设，足细胞hic-5表达的持续局灶节段性肾小球硬化症（FSGS）赋予衰老表型，从而促进进展为终末期肾病。为了验证这一假设，我们提出了三个具体目标。第一个目标将定义hic-5增加足细胞与ECM的粘附从而改变细胞运动性、存活和衰老的分子机制。具体目标二旨在测试小鼠中足细胞特异性hic-5表达的诱导是否引起蛋白尿。第三个目标将确定足细胞中hic-5的长期表达是否诱导肥大、衰老和凋亡，从而引起FSGS并进展为ESRD。如果我们的假设是正确的，这里提出的工作将具有广泛的意义，因为它将使我们更好地了解进展性蛋白尿肾病发展的生物学机制，并为治疗策略的发展提供新的靶点。从长远来看，这将使我们能够开发新的足细胞保护疗法，其通过抑制hic-5介导的足细胞与GBM的粘附增加来解决蛋白尿性肾病。这种hic-5阻断化合物还可以通过抑制hic-5介导的足细胞衰老来减缓FSGS向ESRD的进展。 公共卫生相关性：我们的新的初步数据，在这个授权申请中描述，确定了一个hic-5依赖的信号通路，通过调节足细胞粘附到细胞外基质/肾小球基底膜，有助于蛋白尿肾病的发病机制和进展。本申请的目的是确定足细胞中hic-5表达的调节导致蛋白尿的分子机制，从而为临床急需的新型抗蛋白尿治疗方案的开发铺平道路。

DESCRIPTION (provided by applicant): Podocyte foot processes (FPs) and the interposed slit diaphragm (SD) form the final barrier to protein loss, explaining why podocyte injury is typically associated with marked proteinuria. Podocyte dysfunction, represented by FP effacement, disruption of the SD and proteinuria, is often the starting point for progressive kidney disease. Here we propose to test our central hypothesis that the induction of hic-5 expression in podocytes contributes to the pathogenesis of proteinuria by increasing the adhesiveness of podocyte to the extracellular matrix (ECM)/glomerular basement membrane (GBM). We further hypothesize that the persistence of podocyte hic-5 expression in focal segmental glomerulosclerosis (FSGS) confers a senescence phenotype, thereby promoting the progression to ESRD. To test this hypothesis, we propose three Specific Aims. The first Aim will define the molecular mechanism whereby hic-5 increases podocyte adhesion to the ECM, thereby altering cell motility, survival and senescence. Specific Aim two seeks to test whether the induction of podocyte-specific hic-5 expression in mice causes proteinuria. The third Aim will establish whether the prolonged expression of hic-5 in podocytes induces hypertrophy, senescence and apoptosis, thereby causing FSGS and progression to ESRD. If our hypothesis is correct, the work proposed here will have broad significance because it will provide us with a better understanding of the biological mechanism underlying the development of progressive proteinuric kidney diseases and offers a new target for the development of treatment strategies. This should in the long- term enable us to develop novel, podocyte-protective therapies that tackle proteinuric kidney diseases by suppressing the hic-5 mediated increased adhesion of podocytes to the GBM. Such hic-5 blocking compounds may also slow the progression of FSGS to ESRD by inhibiting the hic-5 mediated senescence of podocytes. PUBLIC HEALTH RELEVANCE: Our novel preliminary data, described in this grant application, identified a hic-5- dependent signaling pathway that contributes to the pathogenesis and progression of proteinuric kidney diseases by modulation podocyte adhesion to the extracellular matrix/glomerular basement membrane. The goal of this application is to define the molecular mechanisms whereby the modulation of hic-5 expression in podocytes causes proteinuria, thereby paving the road for the development of novel anti-proteinuric treatment options for which there is a critical need in the clinic.