NIAMS Light Imaging Facility

在过去一年中，来自13个科、实验室或分支机构的研究人员使用了该设施，21名研究人员新近接受了使用一种或几种仪器的培训。与以往一样，地雷影响调查工作人员协助已经受过培训的设施用户熟悉TIRF等要求更高的技术，以提高图像质量，并准备这些图像供出版。参考书目中列出了从此类帮助中受益的NIAMS出版物和/或显示我们仪器上收集的图像。 在过去的一年里，在LIS进行的显微镜检查有助于扩大NIAMS几个研究目标领域的知识： i）了解免疫系统及其疾病（Suzuki等，2010）; ii）了解皮肤发育和疾病（Duverger等人，2011; Hwang等人，2011年）; iii）了解肌肉发育（Juan等人，2011）和肌肉疾病（拉本等人，2010 a和B; Takikita等，2010; Xu等，2010）。 NIAMS研究人员可用的显微镜技术的范围已经扩大了一个新的物镜透镜，徕卡25 × 1.0，高数值孔径，长工作距离的物镜，是特别有用的活体显微镜。 在国会助理代表团访问和骨科医生学会访问期间，设施工作人员展示了显微镜技术的使用及其在NIAMS目标区域的应用，在此期间，骨科患者及其医生参观了设施。

Researchers from 13 Sections, Laboratories, or Branches have used the Facility during the past year and 21 researchers have been newly trained to use one or several of the instruments. As always, LIS staff have assisted already trained Facility users to get familiar with the more demanding techniques such as TIRF, to improve the quality of their images, and to prepare these images for publication. NIAMS publications which have benefited from such help and/ or show images collected on our instruments are listed in the bibliography. Microscopy done in LIS in the past year has contributed to expand knowledge in several NIAMS research target areas: i) understanding the immune system and its diseases (Suzuki et al, 2010); ii) understanding skin development and diseases (Duverger et al., 2011; Hwang et al., 2011); iii) understanding muscle development (Juan et al., 2011) and muscle diseases (Raben et al., 2010 a and b; Takikita et al, 2010; Xu et al, 2010). The reach of the microscopy techniques available to NIAMS researchers has been expanded by the addition of a new objective lens, the Leica 25x 1.0, a high numerical aperture, long working distance objective that is particularly useful for intravital microscopy. Facility Staff have demonstrated the use of microscopy techniques and their applications to NIAMS target areas during a visit by a delegation of Congressional Aides and a visit by the Academy of Orthopedic Surgeons, during which orthopedic patients and their physicians toured the Facility.