SIRNA AND DEVELOPMENT OF A MALE CONTRACEPTIVE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 这项试点提案的具体目的是确定狒狒睾丸中Ldhc的敲低是否会破坏精子功能，并作为男性避孕技术的基础。Goldberg和同事（Odet等人，Biol Reprod. 2008年7月; 79（1）：26-34。Epub 2008 Mar 26）成功地靶向破坏小鼠中的Ldhc基因。 这些雄性的精子不能使卵子受精。 这一观察结果证实LDHC是避孕发展的一个目标。 我们的合作者，耶鲁大学的Mark Saltzman博士已经能够证明siRNA可以用于生物可降解的递送系统中以敲低基因表达（个人交流），这表明以这种方式靶向Ldhc应该是一种有效的男性避孕药。 显然需要为男子提供创新的避孕技术。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The specific aim of this pilot proposal is to determine whether knockdown of Ldhc in the baboon testis can disrupt sperm function and serve as the basis for a male contraceptive technology. Goldberg and co-workers (Odet et al., Biol Reprod. 2008 Jul; 79(1):26-34. Epub 2008 Mar 26) were successful in targeting disruption of the Ldhc gene in mice. Sperm from these males were unable to fertilize eggs. This observation validates LDHC as a target for contraceptive development. Our collaborator, Dr. Mark Saltzman, Yale University has been able to demonstrate that siRNA can be utilized in a biodegradable delivery system to knock down gene expression (personal communication) suggesting that targeting Ldhc in this way should be an effective male contraceptive. There is an obvious need for innovative contraceptive technology for men.