IMAGING OF MOUSE LUNG TUMORS WITH FLUORESCENCE TOMOGRAPHY / MICRO-CT

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 荧光断层扫描（FT）是一种高灵敏度的非侵入性临床前成像方法。随着我们最近方法学发展的成功，我们正在将新开发的技术转化为肺癌小鼠的临床前体内成像。我们以前成功地进行了研究，使用相同的动物模型与micro-CT，其中血管化的肿瘤显着增强，由于泄漏的血管在肿瘤。在拟议的研究中，我们计划测试荧光造影剂可以通过相同的机制用于增强肿瘤的假设。此外，由于生物分布的差异，我们还将比较荧光和X射线造影剂在肿瘤增强方面的差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Fluorescence tomography (FT) is a highly sensitive noninvasive method for preclinical imaging. With the success in our recent methodological development, we are translating the newly developed technology to preclinical in vivo imaging of mice with lung cancers. We have previously successfully conducted studies using the same animal model with micro-CT, in which vascularized tumors were significantly enhanced due to leakiness of the vasculature in the tumors. In the proposed study, we plan to test the hypothesis that fluorescent contrast agent can be used to enhance tumors via the same mechanism. In addition, due to the differences in biodistribution, we are also going to compare the differences in tumor enhancement using fluorescent and x-ray contrast agent.