AIR-CONTAINING LIPOSOMES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 脂质体是一种自形成的脂质双层阵列，将水的内部隔间与主体水相分离，具有明显的亲水和疏水区域。最初，脂质体制剂 为了用作超声成像增强的靶向造影剂，对其进行了修饰以允许气体包裹。这些制剂被称为“回声脂质体(ELIP)”。当一种气体是 由于热力学原因，气体被封装到脂质体中，可以推定其存在于脂质体双层的两个单分子层之间，或者作为单层覆盖的气泡存在于水中 脂质体隔室。 我们的样品是0.32M甘露醇溶液中的脂质体。脂质体的平均粒径分布为100 nm和1000 nm两个主要组分，平均粒径为800 nm。 在我们之前的研究中，我们成功地将药物和基因共包裹到Elip中，并使用冷冻断裂电子显微镜对我们的Elip进行了成像。高声波脂质体已经证明了一种 球形结构，具有声学配方。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Liposomes are self-forming lipid bilayer arrays separating an aqueous internal compartment from the bulk aqueous phase with clearly separated hydrophilic and hydrophobic regions. Initially, liposomal preparations were modified to permit gas encapsulation in order to be used as a targetable contrast agent for ultrasound imaging enhancement. These preparations have been termed "echogenic liposomes (ELIP)". When a gas is encapsulated into a liposome, the gas can be presumed, for thermodynamic reasons, to reside between the two monolayers of the liposome bilayer or as a monolayer covered air bubble within the aqueous compartment of liposomes. Our samples are liposomes in 0.32 M mannitol solution. The size distribution of of the liposomes falls into two primary populations, 100 nm and 1,000 nm, with the average size at 800nm. In our previous research, we have successfully co-encapsulated drugs and genes into ELIP and have imaged our ELIP using freeze fracture electron microscopy. The highly acoustic liposomes have demonstrated a spherical structure with the acoustic formulation.