INTERNATIONAL GENE TRAP CONSORTIUM (IGTC) RESOURCE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 随着人类和小鼠基因组序列的完成，人们对开发小鼠全基因组突变工具的兴趣越来越大，目标是在完整生物体的背景下分析基因组功能。 基因捕获是一种高通量的方法，可用于在小鼠胚胎干（ES）细胞的基因组中引入插入突变。基因捕获载体同时突变和报告内源基因在插入位点的表达，并提供DNA标签用于快速鉴定破坏的基因。从携带基因陷阱插入的ES细胞系产生突变小鼠可应用于大规模哺乳动物基因的功能分析。 国际陷阱联盟（IGTC）由世界各地的实验室组成，共同努力产生突变小鼠ES细胞系的公共文库。这些细胞系可以由对在小鼠中产生标记的功能丧失突变感兴趣的科学家在非合作的基础上获得。除了功能丧失之外，较新的基因捕获载体提供了多种插入后修饰策略，以允许产生其他实验等位基因。 IGTC的合作目标是产生代表小鼠基因组中所有或大多数基因的国际资源，并提供生物信息学和后勤支持，使资源对科学家有价值和可用。 在2005年4月于UCSF举行的IGTC研讨会上，IGTC的成员同意通过为IGTC开发一个面向用户的网站来集中访问所有公开可用的基因捕获线。 还一致认为，应该有标准化的注释和鉴定，以提供基因捕获线的高置信度数据。 IGTC网站、数据库、注释和标识管道由RBVI托管。 IGTC数据库和网站的托管转移到位于戴维斯的加州大学的小鼠生物学项目，自2010年10月1日起生效。 有关该项目的更多信息，请访问http://www.genetrap.org/

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. With the completion of the human and mouse genome sequences, there has been increased interest in developing tools for genome-wide mutagenesis in the mouse, with the goal of analyzing genome function in the context of the intact organism. Gene trapping is a high-throughput approach that can be used to introduce insertional mutations across the genome in mouse embryonic stem (ES) cells. Gene trap vectors simultaneously mutate and report the expression of the endogenous gene at the site of insertion and provide a DNA tag for the rapid identification of the disrupted gene. The generation of mutant mice from ES cell lines carrying gene trap insertions could be applied to large-scale functional analysis of mammalian genes. The International Trap Consortium (IGTC) consists of laboratories around the world working together to generate a public library of mutated murine ES cell lines. Such cell lines can be obtained on a non-collaborative basis by scientists interested in generating reporter-tagged, loss-of-function mutations in mice. In addition to loss of function, newer gene trap vectors offer a variety of post-insertional modification strategies to allow for the generation of other experimental alleles. The cooperative goal of the IGTC is to generate an international resource representing all or most genes in the mouse genome, and to provide the bioinformatics and logistical support to make the resource valuable and available to scientists. At the IGTC workshop held at UCSF in April, 2005, the membership of the IGTC agreed to centralize access to all publicly available gene trap lines by developing a user-oriented Website for the IGTC. It was further agreed that there should be a standardized annotation and identification to provide high confidence data for gene trap lines. The IGTC website, database, and annotation and identification pipeline are hosted by the RBVI. Hosting of the IGTC database and web site was transferred to the Mouse Biology Program at the University of California at Davis, effective October 1, 2010. Further information on this project is available at http://www.genetrap.org/