METAL NANOPARTICLES AS IMAGING, TARGETING, & THERAPEUTIC AGENTS FOR CANCER

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 金属纳米粒子（NP）由于其高度理想的光谱和分子特性，作为一种新型的癌症成像和治疗的综合平台而受到关注。虽然已经非常详细地研究了大分子抗癌药物的靶向，但目前对金属纳米颗粒靶向的动力学知之甚少，主要是由于缺乏体内监测它们的技术。拟议项目将开发调制成像（MI）作为三维定量成像工具，以监测大块组织中的NP（目标1）。我们将在体内组织模拟模型和小鼠肿瘤异种移植物中验证该系统。该系统将用于研究NP肿瘤靶向的动力学（目的2）。特别是，我们将研究纳米颗粒的大小，形状和靶向策略（即被动与配体介导）对小鼠肿瘤中的积累速率和亲和力的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Metal nanoparticles (NP) have gained interest as a novel platform for integrated cancer imaging and therapy due to their highly desirable spectral and molecular properties. While the targeting of macromolecular anti-cancer drugs has been studied in great detail, there is currently little known about the dynamics of metal nanoparticle targeting primarily due to the lack of techniques to monitor them in vivo. The proposed project will develop Modulated Imaging (MI) as a three dimensional quantitative imaging tool to monitor NP's in bulk tissue (Aim 1). We will validate this system in tissue simulating phantoms and in murine tumor xenografts in vivo. This system will be used to study the kinetics of NP tumor targeting (Aim 2). In particular, we will study the effect of nanoparticle size, shape, and targeting strategy (i.e. passive vs. ligand-mediated) on the accumulation rate and affinity in murine tumors.