COMBINED LASER AND TOPICAL ANTIANGIOGENIC THERAPY OF PORT WINE STAIN

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在激光照射的皮肤中，与伤口愈合反应相关的血管生成可以导致光凝血管的再生，从而降低血管病变的治疗结果，如鲜红斑点胎记。我们的具体目标是通过动物模型确定联合使用激光和外用雷帕霉素是否会导致长期的血管切除。啮齿动物窗口室模型(RWCM)中的血管将被多个激光脉冲照射，以确保可靠和密集的光凝。血管的进展将通过彩色数字摄影和激光散斑成像来记录。一组RWCM仅用激光照射，另一组RWCM用激光照射，并用抗血管生成剂雷帕霉素(RPM)涂于表皮侧，为期14天。我们将比较这两组光凝血管的再生情况，以确定外用雷帕霉素的效果。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Angiogenesis associated with the wound healing response in laser-irradiated skin can cause regeneration of the photocoagulated blood vessels and thus reduce the therapeutic outcome of vascular lesions such as port wine stain birthmarks. Our specific aim is to determine whether the combined use of laser and topical rapamycin will induce long-term blood vessel removal using animal models. Blood vessels in the rodent window chamber model (RWCM) will be irradiated with multiple laser pulses to ensure reliable and intensive photocoagulation. The progression of the blood vessels will be recorded by color digital photography and laser speckle imaging. One group of RWCM will be irradiated with laser only and another group of RWCM will be irradiated with laser and an anti-angiogenic agent, rapamycin (RPM) will be applied to the epidermal side for 14 days. We will compare the regrowth of photocoagulated blood vessels in these two group to determine the effect of topical rapamycin.