INDOCYANINE GREEN PHOTODYNAMIC THERAPY FOR VASCULAR LESIONS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 本实验旨在评价ICG-PDT对小鼠窗房血管系统(一种模拟PWS血管系统的动物模型)的影响。具体地说，我们感兴趣的是确定ICG-PDT实现的血管关闭水平。小鼠将接受ICG，然后进行激光照射。ICG将被逆行注射，使ICG扩散到血液中。延迟1分钟后，用GentleLase 755 nm紫翠宝石激光照射皱纹的表皮侧；脉冲宽度和光斑大小分别为3ms和10 mm。然后，小鼠将暴露在30、40或50J/cm2的辐射照射下。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our experiments aim to evaluate the effect of ICG-PDT on mouse window chamber vasculature (an animal model which simulates PWS vasculature). Specifically, we are interested in determining the level of vascular shutdown achieved with ICG-PDT. Mice will receive ICG followed by laser irradiation. ICG will be injected retroorbitally allowing the ICG to diffuse into the bloodstream. Following a 1-minute delay, the epidermal side of the skin-fold will be irradiated with a GentleLASE 755 nm alexandrite laser; pulse duration and spot size will be 3 ms and 10 mm respectively. Mice will then be exposed to radiant exposures of 30, 40 or 50 J/cm2.