STRUCTURAL STUDIES OF THE EUKARYOTIC TRANSCRIPTION MACHINERY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 转录是基因表达途径的第一步，也是关键的控制点。转录调控是发育、肿瘤发生和其他基本过程的基础。在真核生物中，核糖核酸聚合酶II(PolI)负责信使RNA的转录，使Polo？S调控成为基因表达的中心。在本提案期间，我们将继续对刑警组织转录机制的结构进行研究。这些研究目前正沿着三个途径进行：第一，使用突变分析详细分析延长的PolII；第二，PolII与起始和延长因子的共晶；第三，对介体复合体的结构研究，这是一种POL对调节蛋白做出反应所需的22个多肽复合体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcription is the first step and the key control point in the pathway of gene expression. Transcriptional regulation underlies development, oncogenesis, and other fundamental processes. In eukaryotes the enzyme RNA Polymerase II (pol) is responsible for transcription of messenger RNA making pol?s regulation central to gene expression. During the period of this proposal we will continue our structural studies of the pol transcription machinery. These studies are currently proceeding along three avenues: first, detailed analysis of the elongating pol II using mutation analysis; second, co-crystals of pol II with initiation and elongation factors; third, structural studies of the mediator complex, a 22 polypeptide complex required for pol to respond to regulating proteins.