STRUCTURAL AND FUNCTIONAL STUDIES OF ION CHANNELS AND ION CHANNEL DOMAINS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 大脑和心脏中的电信号是由离子通道蛋白的作用驱动的。这些蛋白质来自细胞膜上的孔洞，这些孔洞的打开和关闭允许钙和钾等离子通过。我们正在研究一系列离子通道、离子通道调节蛋白和转运蛋白的结构和功能。我们的目标是揭示这些跨膜蛋白工作的基本生物物理机制，了解它们在原子细节上的结构和相互作用，并了解它们在人类疾病中的功能如何受到损害，如心律失常、癫痫、耳聋和精神疾病。对于我们的研究，了解我们研究的蛋白质和蛋白质片段的分子同一性是必不可少的。质谱学为这些分子鉴定提供了最准确的方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Electrical signaling in the brain and heart is driven by the action of ion channel proteins. These proteins from holes in the cell membrane that open and close to allow the passage of ions such as calcium and potassium. We are investigating the structures and functions of a range of ion channels, ion channel regulatory proteins, and transporters. The goals are to uncover the basic biophysical mechanisms by which these transmembrane proteins work, to understand their structures and interactions in atomic detail, and to understand how their functions are compromised in human diseases such as cardiac arrhythmias, epilepsy, deafness, and mental illness.For our research it is essential to know the molecular identity of the proteins and protein fragments that we study. Mass spectrometry provides the most accurate way to make these molecular identifications.