REGULATION OF RAS/MAPK SIGNALLING BY SPREDL AND RELATED PROTEINS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 Spredl，与ENA VASP同源结构域相关的Sprouty，似乎是Ras/MAPK途径的拮抗剂，尽管它如何抑制该途径尚不确定。 完成Spredl的串联亲和纯化，然后进行质谱分析，将使我们能够发现Spredl的新的结合伴侣，其可能在Ras/MAPK途径的抑制中发挥作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Spredl, Sprouty related with an ENA VASP homology domain, appears to be an antagonist of the Ras/MAPK pathway, although how it inhibits the pathway is uncertain. Completing a tandem affinity purification of Spredl, followed by mass spectrometry, will allow us to uncover novel binding partners of Spredl that may play a role in the inhibition of the Ras/MAPK pathway.