FASTER IDENTIFICATION OF EPIDEMIC BACTERIAL PATHOGENS ON THE US-MEXICAN BORDER

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 多重抗生素耐药性、耐甲氧西林金黄色葡萄球菌（MRSA）是感染患者的主要威胁。在全球范围内由MRSA引起的大多数感染是由某些克隆引起的，通常被称为“流行性MRSA”。鉴定感染流行性MRSA克隆的患者，并对相关病房和区域应用严格的屏障技术和接触隔离，可以阻止院内MRSA爆发，降低总体发病率和死亡率。在这个试点项目中，我们打算： 1. 确定拉斯克鲁塞斯地区MRSA克隆的分子身份; 2. 与已建立的MRSA分子分型技术相比，确定高灵敏度流式细胞术（FCM）在真实的环境中确定MRSA菌株相关性的有效性; 3. 建立Paso Del Norte地区MRSA菌株的自然历史文库，与世界各地的研究人员进行交流，并进一步研究新的MRSA克隆引入和/或当地地理演变; 4. 为在美墨边境建立细菌病原体分型中心奠定科学基础。 我们假设，一个独特的流式细胞术（FCM）为基础的技术开发的LANL将快速区分MRSA菌株以及两个金标准分子分型技术，并在更短的时间。证明FCM技术是确定MRSA菌株分子身份和相关性的有效工具，将有助于使该技术合法化，并允许FCM提高鉴定流行性MRSA克隆和其他致病性细菌克隆的速度。此外，在我们区域建立一个强有力的分子流行病学/细菌分型单位，理论上可以承担查明潜在细菌生物武器爆发的责任，并用于在较短时间内追踪这种感染的来源。此外，这项试点研究将在LANL的Babetta Marrone实验室（正在开发尖端细菌分型/鉴定技术）和NMSU教师之间建立牢固的关系，其中一些技术可以在真实的世界环境中进行测试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Multiple-antibiotic-resistant, methicillin-resistant Staphylococcus aureus (MRSA) presents a major threat to infected patients. The majority of infections caused by MRSA on a global scale are caused by certain clones, often referred to as "epidemic MRSA". The identification of patients infected with epidemic MRSA clones and application of stringent barrier techniques and contact isolation to the associated wards and areas, can lead to a halt in nosocomial MRSA outbreaks and reduce overall morbidity and mortality. In this pilot project we intend to: 1. Determine the molecular identity of MRSA clones in the Las Cruces region; 2. Determine the effectiveness of high-sensitivity flow cytometry (FCM) in determining MRSA strain relatedness in a real world setting, in comparison to established MRSA molecular typing techniques; 3. Produce a natural history library of MRSA strains from the Paso Del Norte region for exchange with researchers around the world and further examination of novel MRSA clone introductions and/or local geographical evolution; and 4. Develop the scientific foundation for the development of a bacterial pathogen-typing center on the US-Mexican border. We hypothesis that a unique flow cytometry (FCM)-based technique developed at LANL will comparably differentiate MRSA strains as well as two gold standard molecular typing techniques, and in less time. Proof that the FCM technique is an effective tool for determining MRSA strain molecular identity and relatedness, will help legitimize this technology and allow FCM to increase the speed at which epidemic MRSA clones and other pathogenic bacterial clones are identified. Furthermore, establishment of a strong molecular epidemiology/bacterial typing unit in our region could theoretically be called into duty to identify outbreaks of potential bacterial bioweapons and used to track the source of such infections in a relatively short time. In addition, this pilot study will establish a firm relationship between the laboratory of Babetta Marrone at LANL which is developing cutting edge bacterial typing/identification technology and NMSU faculty where some of these techniques can be tested in a real world setting.