FASTER IDENTIFICATION OF EPIDEMIC BACTERIAL PATHOGENS ON THE US-MEXICAN BORDER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 耐多重抗生素、耐甲氧西林的金黄色葡萄球菌(MRSA)是感染患者的主要威胁。在全球范围内，MRSA造成的大多数感染是由某些克隆人引起的，通常被称为“流行性MRSA”。确定感染流行的MRSA克隆的患者，并在相关病房和地区应用严格的屏障技术和接触性隔离，可以导致医院内MRSA暴发的停止，并降低总体发病率和死亡率。在这个试点项目中，我们打算： 1.确定拉斯克鲁塞区MRSA克隆的分子同源性； 2.与已建立的MRSA分子分型技术比较，确定高灵敏度流式细胞术(FCM)在真实环境中确定MRSA菌株相关性的有效性； 3.制作北帕索德尔省地区耐甲氧西林金黄色葡萄球菌菌株的自然历史资料库，以便与世界各地的研究人员交流，并进一步研究新的耐甲氧西林金黄色葡萄球菌克隆引进和/或当地的地理进化；以及 4.为在美墨边境建立细菌病原体分型中心奠定科学基础。 我们假设LANL开发的一种独特的基于流式细胞术(FCM)的技术将在更短的时间内区分MRSA菌株和两种金标准分子分型技术。证明FCM技术是确定MRSA菌株分子同一性和亲缘关系的有效工具，将有助于使这项技术合法化，并使FCM能够提高鉴定流行的MRSA克隆和其他致病细菌克隆的速度。此外，在我们区域建立一个强大的分子流行病学/细菌分型股，理论上可以负责查明潜在的细菌生物武器的暴发，并用于在相对较短的时间内追踪这种感染的来源。此外，这项初步研究将在LANL的Babetta Marrone实验室和NMSU教职员工之间建立牢固的关系，该实验室正在开发尖端的细菌分型/鉴定技术，其中一些技术可以在现实世界中进行测试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Multiple-antibiotic-resistant, methicillin-resistant Staphylococcus aureus (MRSA) presents a major threat to infected patients. The majority of infections caused by MRSA on a global scale are caused by certain clones, often referred to as "epidemic MRSA". The identification of patients infected with epidemic MRSA clones and application of stringent barrier techniques and contact isolation to the associated wards and areas, can lead to a halt in nosocomial MRSA outbreaks and reduce overall morbidity and mortality. In this pilot project we intend to: 1. Determine the molecular identity of MRSA clones in the Las Cruces region; 2. Determine the effectiveness of high-sensitivity flow cytometry (FCM) in determining MRSA strain relatedness in a real world setting, in comparison to established MRSA molecular typing techniques; 3. Produce a natural history library of MRSA strains from the Paso Del Norte region for exchange with researchers around the world and further examination of novel MRSA clone introductions and/or local geographical evolution; and 4. Develop the scientific foundation for the development of a bacterial pathogen-typing center on the US-Mexican border. We hypothesis that a unique flow cytometry (FCM)-based technique developed at LANL will comparably differentiate MRSA strains as well as two gold standard molecular typing techniques, and in less time. Proof that the FCM technique is an effective tool for determining MRSA strain molecular identity and relatedness, will help legitimize this technology and allow FCM to increase the speed at which epidemic MRSA clones and other pathogenic bacterial clones are identified. Furthermore, establishment of a strong molecular epidemiology/bacterial typing unit in our region could theoretically be called into duty to identify outbreaks of potential bacterial bioweapons and used to track the source of such infections in a relatively short time. In addition, this pilot study will establish a firm relationship between the laboratory of Babetta Marrone at LANL which is developing cutting edge bacterial typing/identification technology and NMSU faculty where some of these techniques can be tested in a real world setting.