FASTER IDENTIFICATION OF EPIDEMIC BACTERIAL PATHOGENS ON THE US-MEXICAN BORDER

更快地识别美墨边境的流行性细菌病原体

• 批准号: 7724247
• 负责人: JOHN E GUSTAFSON
• 金额: $ 1.61万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724247
• 关键词: Antibiotic Resistance; Area; Bacterial Typing; Computer Retrieval of Information on Scientific Projects Database; Development; Disease Outbreaks; Effectiveness; Epidemic; Evolution; Faculty; Flow Cytometry; Foundations; Funding; Gold; Grant; Infection; Institution; Laboratories; Lead; Libraries; Mexican; Molecular; Molecular Epidemiology; Morbidity - disease rate; Natural History; Patients; Pilot Projects; Research; Research Personnel; Resources; Source; Speed; Standards of Weights and Measures; Techniques; Technology; Testing; Time; United States National Institutes of Health; base; methicillin resistant Staphylococcus aureus; mortality; novel; pathogen; tool; ward

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Multiple-antibiotic-resistant, methicillin-resistant Staphylococcus aureus (MRSA) presents a major threat to infected patients. The majority of infections caused by MRSA on a global scale are caused by certain clones, often referred to as "epidemic MRSA". The identification of patients infected with epidemic MRSA clones and application of stringent barrier techniques and contact isolation to the associated wards and areas, can lead to a halt in nosocomial MRSA outbreaks and reduce overall morbidity and mortality. In this pilot project we intend to: 1. Determine the molecular identity of MRSA clones in the Las Cruces region; 2. Determine the effectiveness of high-sensitivity flow cytometry (FCM) in determining MRSA strain relatedness in a real world setting, in comparison to established MRSA molecular typing techniques; 3. Produce a natural history library of MRSA strains from the Paso Del Norte region for exchange with researchers around the world and further examination of novel MRSA clone introductions and/or local geographical evolution; and 4. Develop the scientific foundation for the development of a bacterial pathogen-typing center on the US-Mexican border. We hypothesis that a unique flow cytometry (FCM)-based technique developed at LANL will comparably differentiate MRSA strains as well as two gold standard molecular typing techniques, and in less time. Proof that the FCM technique is an effective tool for determining MRSA strain molecular identity and relatedness, will help legitimize this technology and allow FCM to increase the speed at which epidemic MRSA clones and other pathogenic bacterial clones are identified. Furthermore, establishment of a strong molecular epidemiology/bacterial typing unit in our region could theoretically be called into duty to identify outbreaks of potential bacterial bioweapons and used to track the source of such infections in a relatively short time. In addition, this pilot study will establish a firm relationship between the laboratory of Babetta Marrone at LANL which is developing cutting edge bacterial typing/identification technology and NMSU faculty where some of these techniques can be tested in a real world setting.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 多抗抗生素的耐甲氧西林金黄色葡萄球菌（MRSA）对感染患者构成了主要威胁。 MRSA在全球范围内引起的大多数感染是由某些克隆引起的，通常称为“流行MRSA”。鉴定感染了流行病MRSA克隆的患者，并应用了严格的障碍技术以及与相关病房和地区的接触隔离，可能会导致医院MRSA暴发的停止，并降低总体发病率和死亡率。在这个试点项目中，我们打算： 1。确定LAS Cruces区域MRSA克隆的分子身份； 2。与已建立的MRSA分子键入技术相比，确定高敏性流式细胞仪（FCM）在确定MRSA菌株相关性方面的有效性； 3。从北北部地区生产MRSA菌株的自然历史库，与世界各地的研究人员进行交流，并进一步研究新颖的MRSA克隆介绍和/或当地的地理演变；和 4。建立科学基础，以开发美国 - 墨西哥边境的细菌病原体型中心。 我们假设在LANL开发的一种独特的基于流式细胞仪（FCM）的技术将相当区分MRSA菌株以及两种金标准分子拼字技术，并且在更少的时间内。证明FCM技术是确定MRSA菌株分子识别率和相关性的有效工具，将有助于使该技术合法化，并允许FCM提高鉴定出流行病的MRSA克隆和其他病原细菌克隆的速度。此外，从理论上讲，在我们地区建立了强大的分子流行病学/细菌分型单元，可以征求义务识别潜在细菌生物武器的暴发，并用于在相对较短的时间内跟踪此类感染的来源。此外，这项试点研究将在Lanl的Babetta Marrone实验室之间建立牢固的关系，该实验室正在开发最先进的细菌打字/识别技术和NMSU教师，其中其中一些技术可以在现实世界中进行测试。