STRUCTURAL STUDIES OF HIGHER ORDER COMPLEXES IN THE GET PATHWAY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们已经解决了真核蛋白复合物的原核同源物的晶体结构，这表明一种新的功能性寡聚体状态。我们已经纯化了一种新的真核蛋白寡聚体，需要确认结构具有相同的形状。我们也有蛋白质与其底物的复合物，这将大大促进我们对蛋白质功能的理解。此外，我们在系统中有多种蛋白质的高阶复合物。所有这些都与生物学相关，我们有许多片段的结构。获得高分辨率的分子包膜将确定相关的蛋白质界面。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have solved a crystal structure of a prokaryotic homologue of a eukaryotic protein complex that suggests a new functional oligomeric state. We have purified the new oligomer of a eukaryotic protein and need to confirm the structures have the same shape. We also have protein in complex with it's substrate that will significantly advance our understanding of the proteins function. In addition, we have higher order complexes of multiple proteins in the system. All of these are biologically relevant and we have structures of many of the pieces. Getting high resolution molecular envelopes will identify relevant protein interfaces.