MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

克莱蒙斯先生建议确定一个小的晶体结构， 核糖体蛋白，S15，来自嗜热脂肪芽孢杆菌， 结晶解析了S15与其配合物的结构， 16S核糖体RNA的最小结合位点， 单独的起始因子If2和与起始因子的复合物。 他 建议设计合适的rRNA作为S15的靶底物 约束力 这将如何实现与S15相同的结合效率 因为天然rRNA没有被清楚地描述。 最后一个目标是 该计划将通过与U博士的合作来实现。 来自麻省理工学院的RajBhanjin，他将提供 甲酰甲硫氨酸带电的起始剂tRNA。 的意义 这些研究将被应用于理解生物学 这些蛋白质的功能没有被描述。

Mr. Clemons proposes to determine the crystal structure of a small ribosomal protein, S15, from Bacillus stearothermophilus, to crystalize and solve the structure of the complex of S15 with its minimal binding site of 16S ribosomal RNA, and to crystalize initiation factor If2 alone and in complex with the initiator. He proposes to design a suitable rRNA as a target substrate for S15 binding. How this will achieve the same binding efficiency for s15 as native rRNA is not clearly described. The last goal of this proposal will be accomplished by a collaboration with Dr. U. RajBhandary from MIT, who will provide purified forymlmethionine charged initiator tRNA. The significance of studies that will be applied for understanding the biological functions of these proteins, is not described.