MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

克莱蒙斯先生建议确定一种小的晶体结构 嗜热脂肪芽孢杆菌核糖体蛋白S15，至 S15与其络合物的结晶及结构解析 16S核糖体RNA的最小结合部位，并结晶 引发因子IF2单独存在，并与引发剂形成复合体。他 建议设计合适的rRNA作为S15的靶底物 有约束力的。这将如何为s15实现相同的绑定效率 由于天然的rRNA没有被清楚地描述。这件事的最后一个目标 提案将通过与U博士的合作完成。 来自麻省理工学院的RajBhandary，谁将提供纯化的 甲硫氨酸荷电启动子tRNA。的重要意义。 将应用于理解生物学的研究 这些蛋白质的功能没有被描述。

Mr. Clemons proposes to determine the crystal structure of a small ribosomal protein, S15, from Bacillus stearothermophilus, to crystalize and solve the structure of the complex of S15 with its minimal binding site of 16S ribosomal RNA, and to crystalize initiation factor If2 alone and in complex with the initiator. He proposes to design a suitable rRNA as a target substrate for S15 binding. How this will achieve the same binding efficiency for s15 as native rRNA is not clearly described. The last goal of this proposal will be accomplished by a collaboration with Dr. U. RajBhandary from MIT, who will provide purified forymlmethionine charged initiator tRNA. The significance of studies that will be applied for understanding the biological functions of these proteins, is not described.