BACTERIOPHAGE P22

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 噬菌体P22是研究最多的沙门氏菌噬菌体之一，一直是 在过去的几十年里，对分子生物学进行了大量的遗传学研究， 其转换和转换能力令人惊叹。在病毒成熟的过程中 成熟的噬菌体，它最初是一种前体，称为proapsid，里面有支架蛋白，然后 DsDNA通过十二个顶点中的一个插入到扩张的衣壳中，并形成支架。 蛋白质就会离开。尾巴附着在这个顶点上，变得成熟并具有感染力。虽然 P22前衣壳和成熟噬菌体的结构都被分解到亚纳米(~9？) )自2003年以来由冷冻-EM解决(酱W.et.等，Nat Struct Biol，2003，10：131-5。)，MOST 由于分辨率较低，对结构细节的了解仍然难以捉摸。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Bacteriophage P22, one of the best studied Salmonella phages, has been the object of tremendous genetic studies for molecular biology in the past several decades and is remarkable for its converting and transducting abilities. During the virus maturation into the mature phage, it starts as a precursor, called procapsid, with scaffolding proteins inside, then the dsDNA inserts into the expanding capsid shell via one of the twelve vertices, and scaffold proteins exit. The tail attaches to this vertex to become mature and infectious. Although both structures of P22 procapsid and mature phage had been solved to subnanometer (~9 ¿ ) resolution by cryo-EM since 2003 (Jiang W. et. al., Nat Struct Biol, 2003, 10:131-5.), most of structural details still remain elusive due to the low resolution.