HIGH SCHOOL MARINE PHAGE PROJECT

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 作为为期8周的高中生暑期实习生计划的一部分，我们开发了一个 研究项目，实习生从几个地点收集海水样本，然后 分析透射电子显微镜图像中是否存在噬菌体。该程序已被开发为 实习生在决定研究计划时是半独立的。一个简单的协议是 随后进行样品准备，离心机将海水过滤到带有碳的网格上 电影。然后使用Leginon和透射电子显微镜对网格进行成像，然后将图像 分析了噬菌体的存在。实习生写了一份报告并做了演示。 实习生与NRAMM的工作人员密切合作，在夏末变得熟悉起来 与科研环境相适应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. As part of an 8 week summer intern program for high school students, we developed a research program where the intern collects sea water samples from several sites and then analyzes TEM images for the presence of phages. The program has been developed so that the intern is semi-independent in determining the research plan. A simple protocol is followed for sample preparation that centrifuges filtered sea water onto a grid with a carbon film. The grids are then imaged using Leginon and the TEM and the images are then analyzed for the presence of phages. The intern writes a report and gives a presentation. The intern works closely with NRAMM staff and by the end of the summer becomes familiar with the scientific research envirnonment.