DIRECT DETECTOR CAMERA EVALUATION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目将评估一台直接探测摄像机的性能，用于单一 用于低温透射电子显微镜的粒子数据采集。直接探测摄像机使用经过辐射加固的 用于直接检测电子的cmos探测器。由于相机没有闪光器， 该相机的MTF特性明显优于传统的CCD相机。 该项目将对这种用于低剂量单粒子的相机设计进行实验评估。 数据收集。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project will evaluate the performance of a direct detection camera for use for single particle data collection for CryoTEM. A direct detection camera uses a radiation hardened CMOS detector for direct detection of electron. Since the camera does not have a scintillator, the MTF characterstics of the camera are significantly better than traditional CCD cameras. The project will experimentally assess this camera design for use for low dose, single particle data collection.