STRUCTURE, FUNCTION AND REGULATION OF THE IPL1 COMPLEX

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 为了保持遗传的完整性，真核细胞必须在有丝分裂过程中将它们的染色体适当地分离到相反的两极。这一过程中的错误导致非整倍体，这与癌症进展和出生缺陷有关。在哺乳动物中，Aurora B复合体负责检测和纠正有问题的着丝粒/微管附着。 本项目的目的是研究酵母中相当于Aurora B复合体的Ipl1复合体的结构、功能和调控。Ipl1复杂的下游靶点是众所周知的，但负责其激活和定位的机制却知之甚少，因此我专注于Ipl1复合体的上游激活和定位。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. To maintain their genetic integrity, eukaryotic cells must segregate their chromosomes properly to opposite poles during mitosis. Errors in this process give rise to aneuploidy that is implicated in cancer progression and birth defects. In mammals, Aurora B complex is responsible for detection and correction of faulty centromere/microtubules attachments. The aim of this project is to study the structure, function and regulation of the Ipl1 complex in yeast that is the equivalent of the Aurora B complex. Ipl1 complex downstream targets are pretty well known but the mechanisms responsible for its activation and localization are poorly understood, so I am focusing on upstream activation and localization of Ipl1 complex.