INTERACTIONS BETWEEN PHOSPHO-ACCEPTING AND KINASE DOMAIN OF ECOLI CHEA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 激活区P4将一个磷酸基团转移到组氨酸激酶CHEA的P1区的保守组氨酸残基上。在这项工作中，我们的目标是确定这两个领域之间的相互作用。以往的实验已经证明，P1结构域彼此之间存在很大的分离，并具有相当大的自由度。为了解决相关的长距离，我们使用了来自E.Coli的氚蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The kinase domain P4, transfers a phosphate group to the conserved histidine residue in the P1 domain of histidine kinase CheA. In this work we aim to determine the interaction between these two domains. Previous experiments have established that P1 domains are widely separated from each other and have considerable degree of freedom. In order to resolve the associated long distances, we use deuterated proteins from E.coli.