PROTEOMICS WITH NANOSPRAY SHEATH FLOW CE-MS INTERFACE AND FAST SCANNING MS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在本世纪初，CE-MS蛋白质组学实验的限制因素是扫描仪器速度慢，跟不上快速高效的CE分离。 我们回顾了童和耶茨在世纪之交进行的原则证明泥坑实验的概念(肛门。化学。1999、71、2270-2278)。我们在LTQ和简化的纳米管套流CE-MS界面上进行了实验，以确定通过将快速CE分离与现代快速扫描MS仪器相结合，是否有可能将快速CE分离作为蛋白质组实验的竞争工具。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Earlier in the decade, the limiting factor in CE-MS proteomic experiments were slow scanning instruments not catching up with fast and efficient CE separation. We revisited the concept of a proof-of principle MUDPIT experiment, performed by Tong and Yates at the turn of the century (Anal. Chem. 1999, 71, 2270-2278). We performed experiments on LTQ and simplified nanospray sheath flow CE-MS interface to see if it is possible to take advantage of fast CE separations as a competitive tool for proteomic experiments by coupling them with modern, fast scanning MS instrumentation.