CHOLESTEROL REMOVAL BY HDL PARTICLES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 -高密度脂蛋白被认为是细胞胆固醇的初始受体，也是反向胆固醇过程的第一步，反向胆固醇过程是胆固醇从细胞膜运输到肝脏进行降解的过程。存在于膜上的胆固醇结构域，即所谓的脂筏，将调节类固醇分子的外流和可及性。 我们用双光子激发的Laurdan GP成像技术研究了这一过程是如何发生的，并将其作为膜系统存在于巨大的单层囊泡和细胞中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. -HDL are considered to be the initial acceptors of cellular cholesterol and the first step in the reverse cholesterol process, the process where cholesterol is transported from the cellular membrane to the liver to be degraded. The existence in the membrane of cholesterol domains, so-called lipd-rafts will regulate the efflux and the accessibility of the sterol molecules. We studied how this process occurs using LAURDAN GP imaging with 2-photon excitation and as a membrane system in Giant Unilamellar Vesicles and cells.