NEW SUBSTRATES FOR FLUORESCENCE MICROSCOPY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 本项目的目的是基于2D-PHC导模的共振激发，设计一种易于实现的、具有高空间分辨率和荧光增强特性的荧光显微镜方案。 众所周知，外部辐射与周期性纳米结构(如PHC)的相互作用会影响电磁波的远场传播。简而言之，这种周期性允许近场中的逝去波与PHC支持的局域泄漏模式的相位匹配。因此，漏模的外部激发与谐振波长的全反射现象有关。然而，光不会突然反射；在PHC和介质(通常是空气或水)之间的界面上存在一个被称为消逝波区的照明区域，其中强度从PHC表面呈指数衰减。利用这种PHC的性质和先前显示的荧光增强[Estrada等人，2010年。光学快报，第18卷，第4期，第3693-3699页(2010)]我们正在设计用于荧光显微镜的新基板。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our purpose in this project is to design an easy-to-implement scheme for fluorescence microscopy with high spatial resolution and fluorescence enhancement properties based on the resonant excitation of 2D-PhC guided modes. It is well known that the interaction of an external radiation with a periodically nanostructure such as a PhC affects the far field propagation of electromagnetic waves. In brief, the periodicity allows for phase-matching of evanescent waves in the near field region to localized leaky modes supported by the PhC. Thus, the external excitation of leaky modes is associated with a total reflection phenomenon for a resonant wavelength. However, the light does not reflect abruptly; and an illuminated region called the evanescent wave region, is present on the interface between the PhC and the medium (typically air or water) where the intensity decays exponentially from the PhC surface. Using this PhC's property and the fluorescence enhancement shown previously [Estrada et al, 2010. Optics Express, Vol. 18, Issue 4, pp. 3693-3699 (2010)] we are designing new substrates for fluorescence microscopy.