Texas Repository for AIDS Neuropathogenesis Research

描述（由申请人提供）：本申请更新德克萨斯神经艾滋病研究中心（TNRC；“Texas”）响应RFAMH-08-021。德克萨斯州是国家神经艾滋病组织联盟（NNTC）的一部分，该联盟包括西奈山医学院、加州大学洛杉矶分校和加州大学圣地亚哥分校的另外3个站点。申请的A部分对所有4个地点都是相同的，包含NNTC的总体目标、运作和共同议程。B部分是站点特定的应用程序。德克萨斯将解决A部分科学议程如下：1)在宿主遗传学中，我们通过测试和构建基于病理的NNTC材料DNA库来启动单元。目前正在合作使用556个DNA分离物，并作为NNTC使用的模板。2)在神经病毒学目标方面，德克萨斯小组准备合作开发尸检脑标本中HIV载量的持久测定方法，该方法将用于NNTC a部分议程。3)在生物标志物领域，德克萨斯州

DESCRIPTION (provided by applicant): This application for renewal of the Texas NeuroAIDS Research Center (TNRC; "Texas") responds to RFAMH-08-021. Texas is part of the National NeuroAIDS Tissue Consortium (NNTC) which includes 3 additional sites at the Mt. Sinai School of Medicine, UCLA, and the UC San Diego. Part A of the application is identical for all 4 sites and contains overall aims, operations and shared agendas of the NNTC. Part B is the Site-specific application. Texas will address the Part A scientific agenda as follows: 1) In host genetics we primed the unit by testing and constructing a pathologically based DNA bank of NNTC materials. Collaboration is underway using 556 DNA isolates, and serves as a template for utilization by the NNTC. 2) For neurovirology aims the Texas unit is primed to collaborate by developing a durable assay of HIV load in autopsy brain specimens that will be used in the NNTC Part A agenda. 3) In the area of biomarkers, Texas will play a prime role in collaborating with the NNTC's need to developing markers of peripheral neuropathy, using results from our unique proteomic analysis of NNTC sensory ganglia. 4) Texas is primed to collaborate in HIV neuropsychiatric comorbidity by expanding upon new insights obtained in an NNTC driven R01 project to elucidate the role of altered dopamine receptors in cocaine use and depression; we will contribute new data and technology to the Part A agenda. 5) Texas is primed to interact in the area of aging comorbidity; techniques were scouted in an NNTC-driven R01 to determine how interferon responses drive protein misfolding and pathological accumulation of brain protein including amyloid. We plan to increase the pool of elderly people in Texas cohort to increase the feasibility of performing translational studies in elderly people with HIV/AIDS. 6) Texas is primed to address discovery of neurochemical breakthroughs because we played a key role in the NNTC gene array project. We will pursue interferon response proteins as biomarkers, which is a key focus of the Part A agenda. 7) Texas is primed to play a part in the drive to delineate the individual effects of gray and white matter pathology on specific neurocognitive domains; we contributed a useful biomarker in white matter to the Part A agenda, which opens the door to further development. 8) Texas will continue to lead the neuropathology quality assurance program of the NNTC in the Part A agenda. These niches conform to certain strengths of Texas that, if exploited fully, can provide breadth and reach to the NNTC agenda. Texas stands ready again to work together to insure that the NNTC remains a vital and lasting national resource in the future. Part A: