UNDERSTANDING MECHANISMS OF hESC SELF-RENEWAL AND CELL FATE COMMITMENT

描述：(申请者提供)：该计划的主要主题将是定义人类胚胎干细胞的基本自我更新机制，以及它们如何被指定为不同的谱系。每个研究项目都是相辅相成的，但具有高度的协同性，并建立在共同的生物学平台上。该计划非常重视使用最新的hESC培养技术，并将与东南科学界进行高度互动。 这3个项目将： -解决早期细胞命运承诺的分子和细胞方面； -确定基因组在分化过程中如何重塑；-以及 -应用最先进的技术来表征hESC的糖基化模式，并 分化的细胞。 这3个核心将： -作为方案和东南部的资源，维护和分发人类可再生能源，提供为期两周的人类可再生能源技术强化培训方案，并作为一般技术资源发挥作用； -建立新技术，以解决制约人类可持续发展领域进展的主要问题； -为该计划提供行政和后勤支持，并充当与东南部互动的渠道。 该计划将极大地加速佐治亚大学和东南部伙伴机构在hESC领域取得的科学进步。总体而言，该团队的发现将对我们对hESC生物学和人类发育的基本理解产生重大影响。所有这些发现将对开发策略至关重要，其中hESCs将用于治疗退行性疾病和修复慢性损伤的细胞疗法。

DESCRIPTION: (provided by applicant): The overarching theme of this Program will be to define basic self-renewal mechanisms of hESCs and how they become specified into different lineages. Each of the research projects is complementary but highly synergistic and built on a common biological platform. The Program places a strong emphasis on using the latest hESC culture technology and will be highly interactive with the Southeast scientific community. The 3 Projects will: - address molecular and cellular aspects of early cell fate commitment; - determine how the genome is remodeled during differentiation;- and - apply state of the art technology to characterize glycosylation patterns in hESC and differentiated cells. The 3 Cores will: - function as a resource for the Program and the Southeast by maintaining and distributing hESCs, providing an intensive 2-week Training Program in hESC technology and functioning as a general technical resource; -establish new technologies designed to solve major problems that restrict progress in the hESC area; - provide administrative, logistical support for the Program and act as a conduit for interactions with the Southeast. The Program will significantly accelerate scientific advances made in the hESC area at the University of Georgia and at partnering Institutions in the Southeast. Overall, discoveries made by this team will have a major impact on our understanding of basic hESC biology and human development. All of these discoveries will be critical for the development of strategies where hESCs are to be used in cell therapies for treating degenerative disease and repair of chronic injury.