Characterization of the LGL Leukemia Virus (PQ 12)

描述（由申请人提供）：该项目的长期目标是鉴定与LGL白血病相关的病毒。该应用程序代表了由一个多学科团队领导的经典和前沿方法的结合，该团队的成员因其对癌症研究和哺乳动物基因组学的个人贡献而受到国际认可。我们提出LGL白血病是由一种与HTLV和HIV相似的新型逆转录病毒引起的。我们将使用经典病毒学方法分离LGL白血病病毒。我们将验证LGL病毒可以从感染细胞传播到靶细胞的假设，并确定高滴度病毒生产的最佳靶点。我们将使用最先进的深度测序结合全基因组分析方法来鉴定LGL白血病病毒序列。

DESCRIPTION (provided by applicant): The long-term goal of this project is to identify the virus associated with LGL leukemia. This application represents a combination of classical and cutting edge approaches led by a multi-disciplinary team whose members are internationally recognized for their individual contributions to cancer research and mammalian genomics. We propose that LGL leukemia is caused by a novel retrovirus with similarities to both HTLV and HIV. We will use classical virology methods to isolate the LGL leukemia virus. We will test the hypothesis that the LGL virus can be transmitted from infected cells to target cells and identify optimum targets for high titer virus production. We will use state-of-the-art deep sequencing combined with whole genome analytical approaches to identify LGL leukemia viral sequences. PUBLIC HEALTH RELEVANCE: This study is investigating the role of a virus in LGL leukemia development. The purpose of this study is to identify the LGL leukemia virus and to understand how it might cause leukemia. This information will make it easier to detect people who are at risk for LGL leukemia, to prevent LGL leukemia and to better treat patients who are affected by the LGL leukemia virus. It will also allow us to determine if the LGL leukemia virus is involved in other cancers and diseases with features that are similar to LGL leukemia.