Proteomics, Metabolomics and Lipidomics

项目摘要(见说明)：蛋白质组学、代谢组学和脂肪组学核心将设在太平洋西北国家实验室(PNNL)。PNNL组学核心测量将基于世界级的基于质谱学(MS)的平台以及独特的能力和资源的可用性。超灵敏蛋白质组学分析将使用精确的质量和时间(AMT)标签方法与高分辨率质谱学相结合，这是一种以小样本量要求提供高基因组覆盖、高定量精度和高灵敏度的过程。全球磷肽测量的量化将使用iTRAQ稳定同位素标记。这一核心的全球代谢组学和脂类组学组件将分别利用GC-MS和LC-MS。这些研究将通过评估直接执行生物功能的广泛的宿主蛋白质、代谢物和脂分子的丰度变化来增强我们对宿主对感染的反应的理解。基于互补转录组学的结果(在此核心之外平行分析)和全球MS-BASE数据或正在研究的生物系统的先验知识，我们还将使用多路目标MS分析来量化定义数量的底物和/或相应编码蛋白质的产物。这些有针对性的蛋白质组学、代谢组学和脂质组学分析将在可行的情况下基于稳定同位素稀释提供准确的绝对定量。

PROJECT SUMMARY (See instructions): The Proteomics, Metabolomics, and Lipidomics Core will be located at Pacific Northwest National Laboratory (PNNL). PNNL OMICs Core measurements will be based upon world-class mass spectrometry (MS)-based platforms and the availability of unique capabilities and resources. Ultra-sensitive proteomics analyses will be performed using the accurate mass and time (AMT) tag approach together with high resolution mass spectrometry, a process that offers high OMIC coverage, high quantitative precision, and high sensitivity with small sample-size requirements. Quantification of global phosphopeptide measurements will be obtained using ITRAQ stable isotope labeling. The global metabolomics and lipidomics components of this Core will utilize GC-MS and LC-MS, respectively. These studies will enhance our understanding of the host response to infection by evaluating changes in the abundance for the broad range of host proteins, metabolites, and lipid molecules that directly carry out biological functions. Based on the results of complementary transcriptomics (analyzed in parallel outside of this Core) and global MS-based OMICs data or a priori knowledge of the biological systems under study, we will also then employ multiplexed targeted MS-based analyses for quantifying a defined number of substrates and/or products of corresponding encoded proteins. These targeted proteomics, metabolomics and lipidomics analyses will provide accurate absolute quantification based on stable-isotope dilution where feasible