A Proteomics Research Resource for Integrative Biology

描述（总体）：精确和准确地测量几乎所有表达的蛋白质及其修饰形式的水平的能力可以为细胞的分子性质和操作、细胞信号传导途径和网络、细胞周期、细胞分化以及与人类健康和各种疾病状态的进展相关的其他过程提供新的见解。该资源中心致力于为NIH支持的生物医学研究社区提供服务，开发和整合用于生物应用的新蛋白质组学技术，传播新技术，并培训科学家使用这些技术。在最初的4年里，资源中心培养了一支强大的研究团队，他们取得了很好的出版记录，一系列富有成效的合作项目，并提供了一系列技术和信息学发展的有效传播。因此，该中心现在准备取得更大的成功。资源中心核心的主要技术目标是开发和应用一套集成的生物学方法，新的分析技术，以及相关的计算和信息学工具，用于比目前可能的更快速，定量，灵敏和全面的蛋白质组学测量应用。资源中心的三个技术核心旨在开发：i）空间和时间蛋白质组学的改进技术，其中包括更好地定义蛋白质的区室定位（主要是相对于膜区室）和量化区室之间蛋白质动力学的方法; 2）蛋白质组的更高通量、更灵敏和更稳健的定量测量，其提供蛋白质及其修饰的更宽的动态范围和更大的覆盖范围;以及3）需要计算和生物信息学工具来提供基于数据的信息，并具有统计上合理的质量衡量标准，以促进新的生物学理解。该中心的技术发展将在一系列具有挑战性的合作应用程序的背景下进行评估，这些应用程序是根据其科学和生物医学相关性以及其从资源中心的能力中受益的能力而选择的。应用范围包括微生物致病性、创伤研究、细胞迁移、肾移植、信号转导、神经蛋白质组学、辐射反应、慢性毒性感染、生物标志物发现和乳腺肿瘤学等领域。

DESCRIPTION (Overall): The ability to precisely and accurately measure levels of nearly all expressed proteins and their modified forms can provide new insights into the molecular nature and operation of cells, cell signaling pathways and networks, the cell cycle, cellular differentiation, and other processes relevant to human health and to the progression of various disease states. This Resource Center is focused on serving the NIH-supported biomedical research community by developing and integrating new proteomic technologies for biological applications, disseminating the new technologies, and training scientists in their use. In its first 4 years the Resource Center has cultivated a strong team of researchers, who have achieved a strong publication record, a productive set of collaborative projects, and provided effective dissemination of an array of technology and informatics developments. As a result the Center is now poised for even greater successes. The primary technology objectives of the Resource Center Cores are to develop and apply an integrated set of biological methods, new analytical technologies, and associated computational and informatics tools, for much more rapid, quantitative, sensitive, and comprehensive proteomics measurement applications than presently possible. The three technological Cores of the Resource Center aim at development of: i) improved technologies for spatial and temporal proteomics, which includes approaches to better define the compartment localization of proteins, primarily with respect to membrane compartments and to quantify the dynamics of proteins between compartments; 2) higher throughput, more sensitive, and much more robust quantitative measurements of proteomes that provide broader dynamic range and greater coverage of proteins and their modifications; and 3) computational and bioinformatics tools needed to provide information based on data with statistically sound measures of quality, so as to facilitate new biological understandings. The Center's technology developments will be evaluated in the context of a range of challenging collaborative applications selected on the basis of their scientific and biomedical relevance, as well as their capacity to benefit from the Resource Center's capabilities. The scope of applications include areas of microbial pathogenicity, trauma research, cellular migration, kidney transplantation, signal transduction, neuroproteomics, radiation response, chronic virulent infections, biomarker discovery, and mammary oncology.