Gametocyte Carriage Rate and Transmission Blocking Vaccine Assay Development

采用巢式病例对照队列设计，对马里Bancoumana疟疾暴露人群的蚊虫摄食测定方法结果与在NIH校园使用培养寄生虫进行的标准膜摄食测定方法进行比较。500名来自Bancoumana村庄的志愿者，年龄从3个月到50岁不等。每月调查疟原虫携带率，包括无性期和配子体期。配子体细胞个体和配子体细胞阴性个体被要求参加用昆虫饲养的蚊子的饲料实验。将这些蚊子的传染性与马里和美国用膜饲养法饲养的蚊子进行了比较。利用寄生虫携带率数据和蚊子感染数据为研究设计提供信息，以评估目标人群的传播阻断疫苗。

Using a nested case-control cohort design, results of mosquito feeding assay methods in a malaria exposed population in Bancoumana, Mali will be compared with standard membrane feeding assay conducted at the NIH campus using cultured parasites. 500 volunteers, ages 3 months to 50 years in Bancoumana villages, are consented and enrolled. Malaria parasite carriage rate, including asexual stage and gametocyte stage are survey at monthly visits. Gametocytemic individuals and gametocyte negative individuals are asked to participate in feed experiments using insectary-raised mosquitoes. Infectivity in these mosquitoes are compared against those of mosquitoes fed in membrane feeding assays in Mali and the USA. The parasite carriage rate data and mosquito infection data are used to inform study design to evaluate transmission blocking vaccine in target populations.