TISSUE CORE FACILITY

项目总结(见说明)： 组织核心(TC)的目的是帮助促进同行评议的科学需求。癌症中心研究人员通过提供一系列集中的服务和技术，专注于高质量、注释丰富的人类生物标本。TC分为四个不同的业务部门，提供收集、处理、注释、储存和最终释放生物检疫所需的端到端生物储存库服务。TC-INTRAGE和采购科负责收集和 用于调查者驱动和通用银行协议的生物标本的初始注释和去鉴定。 TC样品处理实验室提供从血液处理到核酸提取的各种生物样品处理服务。TC-研究组织学服务科通过提供基本的组织学服务、专注于研究的免疫组织化学和构建组织微阵列来支持CCSG的研究人员。TC-Lifetime癌症筛查中心作为该中心终身癌症筛查和预防中心的组织核心卫星设施运行。技术人员负责操作和维护核心仪器和技术。 在上一次赠款期间，Core建立了尖端的科学技术，以支持免疫组织化学(Ventana Discovery Automated System)、自动化、高通量核酸提取系统(Qiagen)，最近还建立了共享仪器Grant资助的自动冷冻系统。还推出了一个新的生物有机磷库存管理系统(LabVantage生物库)。 新的LabVantage生物库系统提供集中的全面样本管理、同意验证和生物量识别，使核心能够有效地充当机构诚实经纪人。生物标本和组织核心服务的获取受到定期更新的生物标本收集和利用政策以及明确定义的按存储容量使用计费机制的管理。 总体而言，所有四个TC部分都被所有5个研究项目大量使用，并为90多个研究人员发起的方案提供支持。核心咨询小组要求CCSG提供400,966美元的支助，这是其业务预算的24%。超过75%的总用户是Moffitt成员并经过同行审查。

PROJECT SUMMARY (See instructions): The purpose of the Tissue Core (TC) is to help the facilitate the scientific needs of peer-reviewed. Cancer Center investigators by providing a collection of centralized services and technologies focused around high quality, richly-annotated human biospecimens. The TC is organized in four distinct operational sections that provide end-to-end biorepository services required for the collection, processing, annotation, storage and ultimate release of biospecimens. The TC-lntake & Acquisition section is responsible for the collection and initial annotation and de-identification of biospecimens for investigator driven and general banking protocols. The TC-Sample Processing Lab provides a wide variety of biospecimen processing services ranging from processing of blood to extraction of nucleic acids. The TC-Research Histology Services section supports CCSG investigators by offering basic histology services, research focused immunohistochemistry and construction of Tissue Microarray. The TC-Lifetime Cancer Screening operates as a Tissue Core satellite facility at the Center's Lifetime Cancer Screening and Prevention Center. The technical staff is responsible for operating and maintaining core instrumentation and technology. Over the last grant period, the Core established sophisticated, state-of-the-science technologies to support Immunohistochemistry (Ventana Discovery Automated System), automated, high-throughput nucleic acid extraction systems, (Qiagen) and most recently a Shared Instrumentation Grant funded automated freezer system. A new biospecimen inventory management system (LabVantage Biobanking) was also launched. The new LabVantage Biobanking system provides centralized comprehensive sample management, consent verification and biospecimen de-identification allowing the core to effectively serve as an institutional Honest Broker. Access to biospecimens and Tissue Core services are governed by regularly updated biospecimen collection and utilization policies and well-defined chargeback mechanism. Collectively, all four TC sections are heavily utilized by all 5 research programs and provide support for over 90 investigator-initiated protocols. The Core requests CCSG Support of $400,966, which is 24% of its operational budget. Over 75% of total users are Moffitt members and peer-reviewed.