Assessing a Novel Paradigm of Inducing Cryotolerance

描述（由申请人提供）：本研究的实际目标是开发和优化具有最低水平冷冻保护剂的无血清冷冻保护培养基。我们打算使用假设驱动的、基于机制的实验方法来进行这些研究，即冷冻保存的成体干细胞（ASCs）在未分化、脂肪细胞或成骨细胞分化状态下的活力可以通过增加热休克蛋白（HSPs）的冷冻前表达来改善。

DESCRIPTION (provided by applicant):The practical goals of this study will be the development and optimization of a serum free cryoprotection medium with minimum levels of cryoprotectants. We intend to pursue these studies using a hypothesis-driven, mechanism-based experimental approach, i.e., the viability of the cryopreserved adult stem cells (ASCs), in the undifferentiated, adipocyte- or osteoblast- differentiated states, can be improved by increasing the pre-freeze expression of heat shock proteins (HSPs).