Properties of concentrated macromolecular solutions

1. 去年报告中描述的粘度计/流变仪已被用于分别测量三种蛋白质（牛血清白蛋白、卵黏液样蛋白和纤维蛋白原）的浓度依赖性粘度，分别用于二元混合物和三元混合物。发现了一种半经验关系，只需说明每种蛋白质的两个参数，就可以定量地说明单个蛋白质和混合物在所有浓度下的粘度，最高可达300 g/l，在实验精度范围内。（格鲁皮和明顿）

1. The viscometer/rheometer described in last year's report has been used to measure the concentration-dependent viscosity of three proteins - BSA, ovomucoid, and fibrinogen - individually, in binary mixtures, and in ternary mixtures. A semi-empirical relation requiring the specification of only two parameters per protein has been found to account quantitatively for the viscosity of individual proteins and mixtures at all concentrations up to 300 g/l to within experimental precision. (A. Grupi & A. Minton) 2. A compact yet extremely precise representation of the radial distribution function of hard sphere fluids and square well fluids has been discovered. This representation will be used to test semiempirical models of the colligative properties of such fluids (T. Hoppe) 3. A compact yet accurate method for coarse-graining electrostatic interactions between proteins has been developed. The novel coarse-graining algorithms will enable Monte Carlo and Brownian Dynamic simulations of the properties of concentrated proteins to be undertaken over a broad range of pH and ionic strength. (T. Hoppe)