MACROMOLECULAR INTERACTIONS SHARED RESOURCE

大分子相互作用共享资源为癌症中心研究人员提供最新进展 用于表征蛋白质-蛋白质、蛋白质-脂肪、蛋白质-核酸和蛋白质-小分子的ART能力 分子相互作用。该设施为大分子的完整表征提供了资源 溶液中的相互作用。这包括对其动力学、热力学和组装状态的描述 互动。表面等离子体共振(SPR)的强度包括结合的测定 动力学和平衡、活性蛋白浓度的测定、分析方法的发展和药物 筛选，结合位点和表位作图。分析超速离心法的优势(8月) 包括表征分子量分布和球化程度的能力 同时测定大分子混合物，并确定单个溶质的部分浓度， 帮助研究构象变化和样品组成，溶液分子质量， 组装的络合物的化学计量，并为自缔合提供严格的热力学 系统。静态和动态光散射(LS)是研究颗粒大小和尺寸的有用工具 细胞、病毒、胶束和蛋白质、大分子组件等大分子的分布， 多糖类和核酸。LS还可用于大分子组装的动力学研究和 实时拆卸。通过在单个共享资源中提供所有这些生物物理工具 设施，我们能够为我们的癌症中心研究人员提供非常高水平的严谨和 用于研究大分子络合物和药物靶标的尖端技术 现代癌症研究的重要组成部分。

The Macromolecular Interactions Shared Resource provides Cancer Center researchers with state-of-the art capabilities for the characterization of protein-protein, protein-lipid, protein-nucleic acid and protein-small molecule interactions. The facility provides resources for the complete characterization of macromolecular interactions in solution. This includes a description of the kinetics, thermodynamics and assembly state of the interaction. The strengths of surface plasmon resonance (SPR) include the determination of binding kinetics and equilibria, the determination of active protein concentrations, assay development and drug screening, and binding site and epitope mapping. The strengths of analytical ultracentrifugation (AUG) include the ability to characterize distributions of molecular weight and degree of globularity for macromolecular mixtures simultaneously, and to determine the partial concentration of individual solutes, aiding in the study of conformational changes and sample composition, solution molecular mass, stoichiometry of assembled complexes, and providing rigorous thermodynamics for self-associating systems. Static and dynamic light scattering (LS) are useful tools in the study of the size and size distribution of cells, viruses, micelles, and macromolecules such as proteins, macromolecular assemblies, polysaccharides, and nucleic acids. LS is also useful for the kinetic study of macromolecular assembly and disassembly in real time. By having all of these biophysical tools available in a single shared resource facility, we are able to offer our Cancer Center researchers an extraordinarily high level of rigor and sophistication for the study of the macromolecular complexes and drug targets that have become such an important part of modern cancer research.