Cellular & Molecular Core - Developmental Exposure Alcohol Research Center

细胞/分子生物学核心为几个主要和试点项目提供了 对他们的建议的最新支持主要是对新鲜(未固定的)标本的评估。这个 由细胞/分子生物学核心提供的具体服务包括(A)提取DMA、RNA、 和来自组织样本的蛋白质，(B)基因分型，实时定量RT-PCR，(C)Western免疫印迹， (D)克隆和合成cRNA核探针；(E)进行放射性和非放射性原位检测 杂交、(F)磷成像分析、(G)基于高效液相的神经化学分析和(H)激光 显微解剖。除了方法和专业知识外，核心还为以下方面提供建议和协作 细胞和分子生物学研究的设计、实现和分析。核心由以下人员指导 米德尔顿博士和瓦拉诺博士的干练监督。通过仔细的咨询和实验设计， 消弧PI系统将受益于统一应用最高级别的质量控制。此外，未加工的 由细胞/分子生物学核心产生的数据将被例行地添加到中央去弧区 数据库，这样任何调查人员都可以分析它们的具体影响和可能与数据的相关性 从他们的研究中产生。细胞/分子生物学核心人员将协助每个 PI在这方面，并将与动物和神经解剖核心密切合作，以确保 根据实验设计，在每次化验中都使用正确的组织样本。

The CELL/MOLECULAR BIOLOGY CORE provides several of the MAIN and PILOT PROJECTS with state-of-the-art support for their proposals primarily in assessments of fresh (unfixed) specimens. The specific services provided by the CELL/MOLECULAR BIOLOGY CORE include (a) extraction of DMA, RNA, and protein from tissue samples, (b) genotyping, real-time quantitative RT-PCR, (c) Western immunoblotting, (d) cloning and synthesis of cRNA riboprobes, (e) performing radioactive and non-radioactive in situ hybridization, (f) phosphorimaging analysis, (g) HPLC-based neurochemical assays, and (h) laser microdissection. In addition to the methodoligcal expertise, the CORE provides advice and collaboration for the design, implementation, and analyses of cell and molecular biology studies. The CORE is directed by the capable oversight of Drs. Middleton and Vallano. Through careful consultation and experimental design, DEARC Pis will benefit from uniform application of the highest level of quality control. Moreover, the raw data generated by the CELL/MOLECULAR BIOLOGY CORE will be routinely added into the central DEARC database, so that any investigator can analyze them for specific effects and possible correlations with data generated from their studies. The CELL/MOLECULAR BIOLOGY CORE personnel will assist each of the Pi's in this regard, and will also work closely with the ANIMAL and NEUROANATOMY CORES to ensure that the correct tissue samples are being utilized in each assay according to the experimental designs.