Metal-titanates as Novel Inhibitors of Cariogenic Biofilms at Tooth-composite Int

描述(申请人提供)：我们本次研究的目标是开发一种新型的抗菌纳米钛-金属络合物。微钛金属络合物已被证明具有抗菌活性。目前，还没有关于纳米钛酸酯与细菌的相互作用以及金属离子Au(III)、Pd(II)和Pt(IV)的抗菌性能的数据。这项研究的中心假设是，将纳米钛酸酯金属络合物加入牙齿粘结树脂中，将抑制牙齿/复合材料修复界面附近的生物膜中致龋菌的生长，而不会影响树脂与牙齿的粘结。这项拟议的工作具有创新性，因为它利用了一类新的钛酸盐-金属络合物作为抗菌剂。此外，与牙齿/复合修复界面相关的生物膜种群动力学将通过单抗共轭量子点进行非侵入性定量。关于细菌生物膜只有三份这样的报告，没有一份适用于致龋菌。拟议的工作组合有望使临床医生能够在最弱的界面上解决复合长寿问题。通过探索钛酸盐-金属络合物与细菌生物膜之间的相互作用，我们将弥合关于一类新型钛酸盐-金属络合物抗菌剂的一项重大知识空白。 公共卫生意义：我们的研究将开发一类新的抗菌纳米钛酸盐-Au(III)、Pd(II)和Pt(IV)的金属络合物。我们将把这种钛酸盐-金属络合物结合到一种牙科粘结树脂中，这种树脂将抑制牙齿/复合材料修复界面附近的生物膜中导致龋齿的细菌生长。我们将用多光子激光显微镜研究在实验室和从受试者身上收集的生物膜。

DESCRIPTION (provided by applicant): Our goal of this investigation is to develop a new class of antibacterial nanoparticle titanate-metal complexes. Micro titanate-metal complexes have been shown to have antimicrobial activity. At present, there are no data on the interactions of nanoparticle titanate with bacteria and on the antibacterial properties of metal ions of Au(III), Pd(II), and Pt(IV). The central hypothesis of this investigation is that nanoparticle titanate-metal complexes that are incorporated into a dental bonding resin will inhibit cariogenic bacterial growth in a biofilm adjacent to the tooth/composite restoration interface without compromising resin-tooth bonding. The proposed work is innovative, because it capitalizes on a new class of titanate-metal complexes as antibacterial agents. In addition, biofilm population dynamics with respect to tooth/composite restoration interface will be quantified non-invasively by Mab-conjugated quantum dots. There are only three such reports on bacterial biofilms and none applied to cariogenic bacteria. The combination of the work proposed is expected to allow the clinicians to tackle the composite longevity problem at the weakest interface. By exploring the interactions between titanate-metal complexes and bacteria biofilm, we will bridge a major gap in knowledge about a new class of titanate-metal complexes antibacterials. PUBLIC HEALTH RELEVANCE: Our investigation will develop a new class of antibacterial nanoparticle titanate-metal complexes of Au(III), Pd(II), and Pt(IV). We will incorporate this titanate-metal complexes into a dental bonding resin, which will inhibit cariogenic bacterial growth in a biofilm adjacent to the tooth/composite restoration interface. We will study the biofilm collected both in the laboratory and from human subjects with multi-photon laser microscopy.