Evaluation of Salmonella in Symptomatic and Asymptomatic Pets_ Study for the VetL

项目摘要 本项目的目标是进一步了解沙门氏菌的流行情况 被感染的动物将通过收集和传播关于 沙门氏菌在有症状和无症状粪便样本中的流行率 动物被送到兽医诊所。虽然宠物可以成为明显生病，由于 沙门氏菌感染，一些宠物可能不会表现出沙门氏菌病的症状，但仍然可以 是携带者，可以感染其他动物或人类。因此，该项目将 以下内容： 阐明宠物沙门氏菌病的低报病例 提供有关宠物沙门氏菌发生的深入信息， 宠物在将这种细菌传播给人类方面所起的作用 为制定有效的教育方法和材料提供关键数据， 预防宠物相关沙门氏菌病 帮助防止沙门氏菌高耐药菌株的进一步出现 将对至少100份有症状样本和100份无症状样本进行检测。粪便样本 将从被带到南达科他州州立大学（SDSU）的宠物身上收集 动物疾病研究诊断实验室（ADRDL）。此外，SDSU扩展 兽医，谁也担任南达科他州的国家公共卫生兽医，将 与全州和邻近地区的兽医和诊所合作， 各国提交研究样品。兽医诊所与重要的同伴 动物病例将被招募用于研究。参与的诊所将获得 样本采集和提交的标准化说明，客户同意书， 标本收集瓶和邮寄材料。该项目将与 沙门氏菌分离、鉴定和分型的有效实验室方法。到 减少获得假阴性结果的风险， 粪便样品，两种选择性富集的组合，并在两种选择性 媒体将进行。随后，沙门氏菌分离株将通过 生化测试还将进行沙门氏菌的血清学鉴定试验。 最后，在亚种水平上进行血清分型和分类时， NVSL（National Veterinary Service Laboratory）所有分离株和结果将 与VetLRN共享。

Project Summary The goal of this project is to gain further insight into the prevalence of Salmonella infected animals. It will be done by collecting and disseminating information on the prevalence of Salmonella in fecal samples from both symptomatic and asymptomatic animals brought to veterinary clinics. While pets can become visibly ill due to Salmonella infection, some pets may not exhibit symptoms of salmonellosis but can still be carriers and can infect other animals or humans. Therefore, this project will do the following: ¿ Illuminate the under-reported cases of pet salmonellosis ¿ Provide in depth information about the occurrence of Salmonella in pets and the role pets play in spreading this bacterium to humans ¿ Provide critical data for developing effective education methods and materials for preventing pet-associated salmonellosis ¿ Help prevent further emergence of highly resistant strains of Salmonella At least 100 symptomatic and 100 asymptomatic samples will be tested. Fecal samples will be collected from pets brought in to the South Dakota State University (SDSU) Animal Disease Research Diagnostic Lab (ADRDL). Additionally, the SDSU Extension Veterinarian, who also serves as South Dakota's state public health veterinarian, will be collaborating with veterinarians and clinics throughout the state and the neighboring states to submit samples for the study. Veterinary clinics with significant companion animal caseload will be recruited for the study. Participating clinics will receive standardized instructions for sample collection and submission, client consent forms, specimen collection vials, and mailing materials. This project will be carried out with efficient laboratory methods for isolation, identification and typing of Salmonella. To diminish the risk of obtaining false negative results, a non-selective pre-enrichment of fecal sample, a combination of two selective enrichments, and plating on two selective media will be performed. Subsequently Salmonella isolates will be confirmed with biochemical tests. Also serological identification tests for Salmonella will be performed. Finally, for serotyping and classification on subspecies level, the isolates will be forwarded to NVSL (National Veterinary Service laboratory). All isolates and results will be shared with VetLRN.