AP-MALDI source with laser post-ionization

描述：利用高分辨率质谱仪在大气条件下进行基于MS的组织成像(IMS)的效率和速度的显著提高将对组织中生物标志物表征的整个分析领域的方法应用产生深远的影响。目前，基于MS的组织成像的效率受到低灵敏度的限制。我们建议使用一个单独的后电离步骤来提高激光从组织中烧蚀的材料的离子产额。为了展示基于所提出的后电离技术的高通量IMS分析，我们计划修改内部构建的紧凑型UV激光系统，该系统能够以非常高的重复频率产生高强度的激光脉冲。

DESCRIPTION: Significant increase in the efficiency and speed of MS-based tissue imaging (IMS) performed at atmospheric conditions using high-resolution mass spectrometer will have a profound impact on method applications to the entire analytical field of characterization of biomarkers in tissues. At present, the efficiency of the MS-based tissue imaging is limited by low sensitivity. We propose to employ a separate post-ionization step to increase the ion yield from the material ablated by laser from the tissues. To demonstrate high-throughput IMS analysis based on the proposed post-ionization technique, we plan to modify the house-built compact UV laser system capable of generation high-intensity laser pulses at very high repetition rate.