Re-wiring of gene expression in the meiotic state

描述（由申请人提供）：概述-这个计划项目探讨的想法，当一个细胞进入减数分裂状态，它经历了基因表达程序的根本重新布线。一个更保守的观点是，减数分裂细胞与营养细胞大致相似，但表达额外的减数分裂特异性基因。我们探索了一个更激进的观点，即使是在营养细胞和减数分裂细胞中表达的基因也以不同的方式表达，在减数分裂细胞中受不同的转录因子调控。此外，减数分裂细胞可能使用比营养细胞更大的机制来控制基因表达。简而言之，当细胞从营养状态分化为减数分裂状态时，它们的基因表达程序被广泛地重新连接。我们将研究这种重新布线和新的减数分裂特异性调节机制。前三个项目使用S.酿酒酵母，并分别集中在早，中，晚减数分裂的调控机制。第四个项目使用S.粟球，并重点研究新的减数分裂调节机制。 项目 1.早期减数分裂程序中基因表达的布线（Futcher博士） 2.减数分裂Cdc 7开关和底物，以及Ndt 80的激活（Dr. Hollingsworth） 3.减数分裂状态下的组蛋白修饰和染色质转换（Neiman和Sternglanz博士） 4.减数分裂中基因调控的新机制：反义RNA和RNA加工。（Dr. Leatherwood） 这些研究项目将得到微阵列核心设施的支持，该设施将在所有四个项目中发挥关键作用。

DESCRIPTION (provided by applicant): Overview - This Program Project explores the idea that when a cell enters the meiotic state, it undergoes a radical re-wiring of gene expression programs. A more conservative view was that meiotic cells are broadly similar to vegetative cells, but express additional, meiosis-specific genes. We explore a more radical view in which even genes that are expressed in both vegetative and meiotic cells are expressed in a different fashion, regulated by different transcription factors in meiotic cells. Furthermore meiotic cells may use a larger repertoire of mechanisms to control gene expression than vegetative cells. In short, when cells differentiate from the vegetative state into the meiotic state, their gene expression programs are extensively re-wired. We will study this re-wiring, and novel, meiosis-specific regulatory mechanisms. The first three projects use S. cerevisiae, and focus on regulatory mechanisms in early, middle, and late meiosis, respectively. The fourth project uses S. pombe, and focuses on novel meiotic regulatory mechanisms. Projects. 1. Wiring of Gene Expression in the Early Meiotic Program (Dr. Futcher) 2. Meiotic Cdc7 switches and substrates, and the activation of Ndt80 (Dr. Hollingsworth) 3. Histone modification and Chromatin Switches in the Meiotic state (Drs. Neiman and Sternglanz) 4. Novel mechanisms of gene regulation in Meiosis: Antisense RNAs and RNA processing. (Dr. Leatherwood) The research Projects will be supported by a Microarray Core Facility, which will play a crucial role in all four projects.