Proteomics Core

生物防御和新发传染病研究计划区域卓越中心， 东北生物防御中心(NBC)组成了一个生物医学研究计划，其核心支持其 多方面的研究和开发活动。作为该计划的一部分，NBC蛋白质组学核心将 在耶鲁大学W.M.凯克基金会生物技术资源实验室内运行，并将 继续提供范围非常广泛的生物分析服务，以支持基础科学、临床前和 美国国家广播公司的临床研究项目。此外，在国家生物防御期或新发传染病时期 疾病紧急情况下，NBC蛋白质组学核心将做好准备，并投入其所有可观的资源 迎接挑战。NBC蛋白质组学核心将从其所有蛋白质组学中提供这些服务 部分：(1)利用最新质量的质谱学和蛋白质图谱资源 光谱仪器(LC-FT-ICR、LC-LTQ-Orbitrap、LC-QTRAP-三重四极杆、MALDI TOF/TOF、LC-QTOF等)和蛋白质图谱技术，如多维蛋白质鉴定 技术(MudPIT)、差分二维(荧光)凝胶电泳(DICE)和多重等压 相对和绝对定量的标记技术(iTRAQ？)。这些技术也将是 与最近建立的磷蛋白图谱方法和其他方法结合使用 蛋白质翻译后修饰表征工具。此外，该资源还将使 其最新开发的临床蛋白质组学工具可用于绝对定量测定 临床相关样本中的生物标志物或已知生物制剂提供给所有NBC调查人员。(2)氨基酸 基于柱后检测的离子交换层析分析。(3)蛋白质 使用Edman(N-末端)测序仪进行测序。(四)提供广泛服务的生物物理科 包括体积排阻色谱/激光散射法测定天然蛋白质 分子量；(5)基于tBOC化学的大规模多肽合成。(6)小分子多肽 基于Fmoc化学和定制的多肽修饰的合成。(7)生物统计组提供 广泛分析蛋白质组数据和改进的量化算法以确定疾病生物标记物 从质谱学数据。凭借其最先进的仪器和非常多样化的蛋白质组 服务，NBC蛋白质组学核心工作人员将与NBC成员协商最佳 最好地支持拟议的研究、实验设计和对产生的数据进行解释的技术 由蛋白质组学核心或其他实验室来满足NBC调查人员的专门需求-世卫组织 将被给予与耶鲁大学调查人员相同的扭亏为盈优先事项

Regional Center of Excellence (RCE) for Biodefense and Emerging Infectious Disease Research program, the Northeast Biodefense Center (NBC) constitutes a biomedical research program with cores supporting its multifaceted research and development activities. As part of this program, the NBC Proteomics Core will operate within the W.M. Keck Foundation Biotechnology Resource Laboratory at Yale University and will continue to provide a very broad range of bioanalytical services to support basic science, preclinical and clinical research programs in the NBC. In addition, in periods of national biodefense or emerging infectious disease emergency, the NBC Proteomics Core will be prepared and devote all of its considerable resources to meeting the challenge. The NBC Proteomics Core will offer these services from all of its proteomics sections: (1) Mass Spectrometry and Protein Profiling resources that utilize state of the art mass spectrometry instrumentation (LC-FT-ICR, LC-LTQ-Orbitrap, LC-QTRAP-Triple Quadrupole, MALDI TOF/TOF, LC-QTOF, etc) and protein profiling technologies like multidimensional protein identification technology (MudPIT), differential 2-D (fluorescence) gel electrophoresis (DICE), and Multiplexed Isobaric Tagging Technology for Relative and Absolute Quantitation (iTRAQ¿). These technologies would also be used in conjunction with the more recently established phosphoprotein profiling methodologies and other protein post translational modification characterization tools. Additionally, this resource will also make available its recently developed clinical proteomics tools for absolute quantitation determination of biomarkers or known biological agents in clinically relevant samples to all NBC investigators. (2) Amino Acid Analysis based on ion-exchange chromatography with post-column ninhydrin detection. (3) Protein Sequencing using Edman (N-terminal) sequencers. (4) A Biophysics Section which offers extensive services including HPLC Size Exclusion Chromatography/laser light scattering determination of native protein molecular weights, (5) Large Scale Peptide Synthesis based on tBOC Chemistry. (6) Small Scale Peptide Synthesis based on Fmoc chemistry with custom peptide modifications. (7) Biostatistics Section offering extensive analysis of proteomic data and improved quantitation algorithms to determine disease biomarkers from mass spectrometric data. With its state-of-the-art instrumentation, and very diverse set of proteomic services, the NBC Proteomics Core staff will consult with members of the NBC regarding the optimum technology to best support the proposed research, experimental design, and interpretation of data produced either by the Proteomics Core or other laboratories to meet the specialized needs of NBC investigators - who will be given the same turn-around priority as Yale investigators