Investigating Astrocyte Diversity and Function in Midbrain Dopaminergic Circuits

在此输入文本，它是您的应用程序的新摘要信息。此部分必须为no 超过30行的文本。 摘要 最近的研究表明，星形胶质细胞在空间上是多样化的，这可能 对患有局部脑功能障碍的精神疾病的后果。然而， 建立星形胶质细胞在脑发育和疾病中的多种功能 由于缺乏特定血统的星形胶质细胞操作工具，这一研究受到了限制。 最近，我们开发了新的转基因方法来标记、纯化和 描述小鼠脑内区域星形胶质细胞群的特征。 中脑和脑干含有腹侧被盖的多巴胺能核。 与精神分裂症、注意缺陷多动障碍有关的黑质和VTA区 和帕金森氏症。我们将检验按地区指定的假设 来自腹侧中脑的星形胶质细胞特别适合支持生存和 来自VTA和SN的多巴胺能投射的连接性。我们将决定 区域限制性中脑星形胶质细胞的分子多样性是迈向 揭示不同区域的星形胶质细胞在神经性疾病中的作用。 可交付的产品包括经过验证的转基因小鼠工具，用于有条件的临时- 中枢神经星形胶质细胞的空间标记、纯化及标记 中脑/后脑的区域异质性星形胶质细胞亚型汇编成 公共数据库。该项目旨在揭示星形胶质细胞的特征。 并提供星形胶质细胞在心理健康中的作用的亚型和洞察。

Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text. ABSTRACT Recent studies indicate that astrocytes are spatially diversified, which may have consequence for psychiatric diseases with regional brain dysfunction. However, establishing diversified functions of astrocytes in brain development and disease has been limited by a lack of tools for lineage-specific astrocyte manipulation. Recently, we developed novel transgenic methods to label, purify and characterize regional astrocyte populations in mouse brain. The midbrain and brainstem contain dopaminergic nuclei of the ventral tegmental area (VTA) and substantia nigra (SN) that are implicated in schizophrenia, ADHD and Parkinson's disease. We will test the hypothesis that regionally-specified astrocytes from the ventral midbrain are uniquely suited to support survival and connectivity of dopaminergic projections from the VTA and SN. We will determine molecular diversity of region-restricted midbrain astrocytes as a first step towards uncovering the role of regionally diverse astrocytes in neurological disorders. Deliverables include validated transgenic mouse tools for conditional temporal- spatial labeling, purification of astrocytes throughout the CNS as well as markers of regionally heterogeneous astrocytes subtypes of mid/hindbrain compiled into a public database. The project is intended to reveal signatures of astrocyte subtypes and provide insight into the role of astrocytes in mental health.